Life Sciences Institute, Zunyi Medical University, Zunyi 563006, PR China.
School of Basic Medical Sciences, Zunyi Medical University, Zunyi 563006, PR China.
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005295.
A novel species of endophytic actinobacterium, designated strain GBK3QG-3, was isolated from surface-sterilized root of collected from Beilun Estuary, Guangxi Zhuang Autonomous Region, PR China. Cells of strain GBK3QG-3 were Gram-stain-positive, aerobic, non-spore-forming and rod-shaped. Growth of GBK3QG-3 was observed at 10-40 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 7.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %). The G+C content of genomic DNA was determined to be 72.2 mol%. On the basis of 16S rRNA gene sequence analysis, strain GBK3QG-3 was most related to the type strain of CCTCC AB 2017058 (97.7 % 16S rRNA gene sequence similarity), followed by SYP-A7303 (97.0 %) and GW-9 (96.9 %). Average nucleotide identity and digital DNA-DNA hybridization values between strain GBK3QG-3 and some strains of the genus with publicly available genomes were below 84.3 and 30.0 %, respectively. The cell-wall peptidoglycan contained ll-diaminopimelic acid. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and an unknown lipid. The major menaquinone was identified as MK-8(H), and the major fatty acids were identified as iso-C, iso-C and anteiso-C. Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, we propose a novel species named sp. nov. The type strain is GBK3QG-3 (=CGMCC 4.7708=JCM 34553).
一株新的内生放线菌，命名为 GBK3QG-3 菌株，从中国广西壮族自治区北仑河口采集的表面消毒的根中分离得到。GBK3QG-3 菌株的细胞为革兰氏阳性、需氧、非孢子形成和杆状。GBK3QG-3 的生长在 10-40°C（最佳，30°C）、pH 5.0-9.0（最佳，pH 7.0）和 0-3%（w/v）NaCl（最佳，0%）存在下观察到。基因组 DNA 的 G+C 含量确定为 72.2 mol%。基于 16S rRNA 基因序列分析，菌株 GBK3QG-3 与 CCTCC AB 2017058 型株（97.7% 16S rRNA 基因序列相似性）最为相关，其次是 SYP-A7303（97.0%）和 GW-9（96.9%）。与具有公开基因组的属内一些菌株的平均核苷酸同一性和数字 DNA-DNA 杂交值分别低于 84.3%和 30.0%。细胞壁肽聚糖含有 ll-二氨基庚二酸。极性脂类包括二磷酸甘油酯、磷脂酰甘油、磷脂酰肌醇、一种未知的磷脂和一种未知的脂类。主要的menaquinone 被鉴定为 MK-8(H)，主要脂肪酸被鉴定为 iso-C、iso-C 和 anteiso-C。基于表型、基因型和系统发育分析收集的证据，我们提出了一个新的物种，命名为 sp. nov.。模式菌株为 GBK3QG-3（=CGMCC 4.7708=JCM 34553）。
