Life Sciences Institute, Zunyi Medical University, Zunyi, 563006, People's Republic of China.
Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning, 530007, People's Republic of China.
Antonie Van Leeuwenhoek. 2021 Apr;114(4):479-486. doi: 10.1007/s10482-021-01535-5. Epub 2021 Mar 1.
A short-rod-shaped, non-spore-forming endophytic actinobacterium, was isolated from a surface-sterilized leaf of Acrostichum aureum in Fangchenggang, Guangxi Zhuang Autonomous Region, China, designated strain CBS4Y-1 and examined by a polyphasic approach to determine its taxonomic position. This actinobacterium was Gram-staining-positive and aerobic. Substrate mycelia and aerial mycelia were not observed, and no diffusible pigments were observed on the media tested. Strain CBS4Y-1 grew optimally with 0-1.0% (w/v) NaCl at 30 °C, pH 7.0-8.0. Comparative analysis of 16S rRNA genes showed that strain CBS4Y-1 shared the highest 16S rRNA gene sequence similarities with Nocardioides marinus CL-DD14 (96.7%) and Nocardioides terrae BX5-10 (96.7%). Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CBS4Y-1 belonged to the genus Nocardioides and formed a distinct cluster within the genus Nocardioides. The DNA G + C content of strain CBS4Y-1 was 71.1 mol%. The cell-wall peptidoglycan contained LL-diaminopimelic acid and MK-8(H) was the predominant menaquinone. Phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylinositol (PI) were detected in the polar lipid extracts. The major fatty acids were iso-C, Cω9c and iso-C. On the basis of phylogenetic analysis, phenotypic and chemotaxonomic characteristics, strain CBS4Y-1 represents a novel species of the genus Nocardioides, for which the name Nocardioides acrostichi sp. nov. is proposed. The type strain is CBS4Y-1 (= KCTC 49238 = CGMCC 4.7548).
从中国广西壮族自治区防城港市的 Acrostichum aureum 表面消毒叶片中分离到一株短杆状、非孢子形成的内生放线菌，命名为菌株 CBS4Y-1，并通过多相方法研究确定其分类地位。该放线菌革兰氏染色阳性，需氧。未见基内菌丝和气生菌丝，在测试的培养基上也未观察到可扩散色素。菌株 CBS4Y-1 在 0-1.0%（w/v）NaCl、30°C、pH7.0-8.0 条件下生长最佳。16S rRNA 基因的比较分析表明，菌株 CBS4Y-1 与 Nocardioides marinus CL-DD14（96.7%）和 Nocardioides terrae BX5-10（96.7%）的 16S rRNA 基因序列具有最高的相似性。基于 16S rRNA 基因序列的系统发育分析和基于核心蛋白质组比对的系统发育分析表明，菌株 CBS4Y-1 属于 Nocardioides 属，并在 Nocardioides 属内形成一个独特的聚类。菌株 CBS4Y-1 的 DNA G+C 含量为 71.1mol%。细胞壁肽聚糖含有 LL-二氨基庚二酸，MK-8（H）是主要的类异戊二烯醌。极性脂提取物中检测到磷脂酰甘油（PG）、双磷脂酰甘油（DPG）和磷脂酰肌醇（PI）。主要脂肪酸为 iso-C、Cω9c 和 iso-C。基于系统发育分析、表型和化学分类特征，菌株 CBS4Y-1 代表 Nocardioides 属的一个新种，命名为 Acrostichum nocardioides sp. nov.。模式菌株为 CBS4Y-1（=KCTC 49238=CGMCC 4.7548）。
