Sui Yan-Bo, Zhang Kui-Kui, Ling Li-Yun, Fan Rui, Liu Li
Department of Cardiology, First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Xiangfang District, Harbin, China.
Department of Cardiology, Heilongjiang University of Chinese Medicine, Xiangfang District, Harbin, China.
Histol Histopathol. 2022 Aug;37(8):803-812. doi: 10.14670/HH-18-454. Epub 2022 Mar 25.
Chronic heart failure (CHF) is a common clinical heart disease. In recent years, traditional Chinese medicines have shown good outcomes in CHF treatment. We aimed to explore the therapeutic effect of Shen Qi Li Xin formula (SQLXF) in CHF. CHF rats were treated with SQLXF at the doses of 8.48, 16.96, and 33.92 g/kg/d once a day for 4 weeks by intragastric administration. The hemodynamic and cardiac function parameters of the rats were monitored by conduction echocardiography. In our results, SQLXF treatment at the doses of 16.96 and 33.92 g/kg/d significantly improved the haemodynamics and cardiac function of CHF rats by enhancing the levels of LVSP, +dp/dtmax, -dp/dtmax, LVEF and LVFS and reducing the levels of LVEDP, LVEDD and LVESD. SQLXF treatment at 16.96 and 33.92 g/kg/d also attenuated the damage of myocardial tissues in CHF rats. In addition, compared with normal rats, the number of pericytes was reduced in myocardial tissues of CHF rats. SQLXF treatment at the doses of 16.96 and 33.92 g/kg/d obviously increased the number of pericytes and proliferation of endothelial cells and promoted angiogenesis in myocardial tissues of CHF rats. In vitro, SQLXF impaired low-oxygen-induced inhibition of cell viability and promotion of apoptosis in primary pericytes. Importantly, SQLXF enhanced the adhesion ability of pericytes to endothelial cells. In conclusion, SQLXF improved myocardial injury in CHF rats by enhancing the interaction between pericytes and endothelial cells, suggesting that SQLXF may be a potential drug for CHF treatment.
慢性心力衰竭（CHF）是一种常见的临床心脏病。近年来，中药在CHF治疗中显示出良好的效果。我们旨在探讨参芪力心方（SQLXF）对CHF的治疗作用。将CHF大鼠分别以8.48、16.96和33.92 g/kg/d的剂量每日灌胃给药1次，持续4周。通过传导超声心动图监测大鼠的血流动力学和心脏功能参数。结果显示，16.96和33.92 g/kg/d剂量的SQLXF治疗可通过提高左室收缩压（LVSP）、最大上升速率（+dp/dtmax）、最大下降速率（-dp/dtmax）、左室射血分数（LVEF）和左室短轴缩短率（LVFS）水平，降低左室舒张末压（LVEDP）、左室舒张末期内径（LVEDD）和左室收缩末期内径（LVESD）水平，显著改善CHF大鼠的血流动力学和心脏功能。16.96和33.92 g/kg/d剂量的SQLXF治疗还可减轻CHF大鼠心肌组织的损伤。此外，与正常大鼠相比，CHF大鼠心肌组织中的周细胞数量减少。16.96和33.92 g/kg/d剂量的SQLXF治疗明显增加CHF大鼠心肌组织中的周细胞数量和内皮细胞增殖，并促进血管生成。在体外，SQLXF可减轻低氧诱导的原代周细胞活力抑制和凋亡促进作用。重要的是，SQLXF增强了周细胞与内皮细胞的黏附能力。总之，SQLXF通过增强周细胞与内皮细胞之间的相互作用改善CHF大鼠的心肌损伤，提示SQLXF可能是一种治疗CHF的潜在药物。
