College of Arts and Sciences, Abu Dhabi University, Abu Dhabi, United Arab Emirates.
Kashmir University, Srinagar, India.
Arch Microbiol. 2022 Mar 25;204(4):219. doi: 10.1007/s00203-022-02826-9.
During past few decades, endophytes have gained importance due their ability to produce bioactive compounds. Many medicinal plants are being exploited for the endophytic isolation to obtain drugs of interest. This study explored the fungal endophytes of Olive (Olea europaea L.) stem from which Fusarium redolens was selected for investigation of bioactive potential. The endophyte was identified using morphological characteristics and internal transcribed spacer ribosomal-deoxyribonucleic acid (ITS-rDNA) sequence analysis. The GCMS analysis of the crude extract yielded chrysophanol and fumaric acid. The culture filtrate of ethyl acetate extract showed significant cytotoxic potential against HepG2 cells, respectively. Furthermore, the screening of antioxidant potential of the ethyl acetate fungal extract using DPPH scavenging assay showed that Fusarium redolens extract exhibited potential activity with a significant EC50 value of 144.7 µg/mL.
在过去的几十年中，由于内生菌能够产生生物活性化合物，因此它们变得越来越重要。许多药用植物被用于内生菌的分离，以获得感兴趣的药物。本研究探索了橄榄（Olea europaea L.）茎中的真菌内生菌，从中选择红镰孢霉进行生物活性潜力的研究。内生菌是通过形态特征和内部转录间隔区核糖体脱氧核糖核酸（ITS-rDNA）序列分析来鉴定的。粗提物的 GCMS 分析得到了大黄酚和富马酸。乙酸乙酯提取物的培养滤液对 HepG2 细胞分别表现出显著的细胞毒性潜力。此外，使用 DPPH 清除试验筛选乙酸乙酯真菌提取物的抗氧化潜力表明，红镰孢霉提取物具有潜在的活性，其 EC50 值为 144.7 µg/mL。
