College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, PR China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, PR China; Shandong Province Collaborative Innovation Center for High-quality and High-efficiency Vegetable Production, Tai'an, 271018, PR China.
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, PR China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, PR China; Shandong Province Collaborative Innovation Center for High-quality and High-efficiency Vegetable Production, Tai'an, 271018, PR China.
Plant Physiol Biochem. 2022 May 15;179:108-119. doi: 10.1016/j.plaphy.2022.03.020. Epub 2022 Mar 22.
Ferredoxin is involved in many biological processes, such as carbon fixation, nitrogen assimilation, chlorophyll metabolism, and fatty acid synthesis, and it plays a role in plant resistance to stress. However, the functions of Fds in peach during stress are unclear. In this study, 11 members of the peach Fd gene family were identified and divided into six groups (I- VI). We carried out bioinformatics analysis on these sequences, analyzed the physical and chemical properties of PpFd protein and the cis-elements in its promoter region, and predicted and compared the differences in gene structure and conserved protein motifs among groups. The results showed that the PpFd protein was highly conserved in plant species. In addition, overexpression of PpFd08 significantly increased the tolerance of transgenic tomato to high-temperature stress. The transcriptome analysis and qRT-PCR results of PpFd08 transgenic apple calli showed that PpFd08 might enhance heat resistance by modulating the expression of heat tolerance related genes. The results of this study provide a new understanding for the further study of the function of PpFd protein in peach and a candidate gene for improving the heat resistance of peach.
铁氧还蛋白参与许多生物过程，如碳固定、氮同化、叶绿素代谢和脂肪酸合成，它在植物抵抗胁迫中发挥作用。然而，Fds 在桃中的功能尚不清楚。本研究鉴定了 11 个桃铁氧还蛋白基因家族成员，并将其分为 6 组（I-VI）。我们对这些序列进行了生物信息学分析，分析了 PpFd 蛋白的理化性质及其启动子区中的顺式元件，并预测和比较了不同组之间基因结构和保守蛋白模体的差异。结果表明，PpFd 蛋白在植物物种中高度保守。此外，PpFd08 的过表达显著提高了转基因番茄对高温胁迫的耐受性。PpFd08 转基因苹果愈伤组织的转录组分析和 qRT-PCR 结果表明，PpFd08 可能通过调节耐热相关基因的表达来增强耐热性。本研究结果为进一步研究 PpFd 蛋白在桃中的功能以及提高桃耐热性的候选基因提供了新的认识。
