State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Nutrients. 2022 Mar 14;14(6):1222. doi: 10.3390/nu14061222.
Atherosclerosis is the main cause of myocardial infarction and stroke, and the morbidity and mortality rates of cardiovascular disease are among the highest of any disease worldwide. Excessive plasma trimethylamine-N-oxide (TMAO), an intestinal metabolite, promotes the development of atherosclerosis. Therefore, effective measures for reducing plasma TMAO production can contribute to preventing atherosclerosis. Probiotics are living microorganisms that are beneficial to the human body, and some of them can attenuate plasma TMAO production. To explore the effects of probiotic supplementation on plasma TMAO in choline-fed mice, we intragastrically administered eight strains of and eight strains of to mice for 6 weeks. Bb4 and BL1 and BL7 significantly reduced plasma TMAO and plasma and cecal trimethylamine concentrations. However, hepatic flavin monooxygenase (FMO) activity, flavin-containing monooxygenase 3 (FMO3), farnesoid X receptor (FXR) protein expression and TMAO fractional excretion were not significantly affected by supplementation. The treatment of strains modulated the abundances of several genera such as UCG-009, UCG-010, which belong to the Firmicutes that has been reported with gene clusters, which may be related to the reduction in intestinal TMA and plasma TMAO. Additionally, a reduction in indicates a reduction in circulating glucose and lipids, which may be another pathway by which strains reduce the risk of atherosclerosis. The effect of strains on also suggests a relationship between the abundance of this genus and TMA concentrations in the gut. Therefore, the mechanism underlying these changes might be gut microbiota regulation. These strains may have therapeutic potential for alleviating TMAO-related diseases.
动脉粥样硬化是心肌梗死和中风的主要原因，心血管疾病的发病率和死亡率在全球所有疾病中是最高的。肠道代谢产物过多的血浆三甲胺氧化物（TMAO）会促进动脉粥样硬化的发展。因此，减少血浆 TMAO 产生的有效措施有助于预防动脉粥样硬化。益生菌是对人体有益的活体微生物，其中一些可以减弱血浆 TMAO 的产生。为了探索益生菌补充对胆碱喂养小鼠血浆 TMAO 的影响，我们将 8 株 和 8 株 灌胃小鼠 6 周。Bb4 和 BL1 和 BL7 显著降低了血浆 TMAO 以及血浆和盲肠三甲胺浓度。然而，肝黄素单加氧酶（FMO）活性、黄素单加氧酶 3（FMO3）、法尼醇 X 受体（FXR）蛋白表达和 TMAO 分数排泄不受 补充的显著影响。 菌株的处理调节了几个属的丰度，如 UCG-009、UCG-010，它们属于Firmicutes，已报道与 基因簇有关，这可能与肠道 TMA 和血浆 TMAO 的减少有关。此外， 减少表明循环葡萄糖和脂质减少，这可能是 菌株降低动脉粥样硬化风险的另一种途径。 菌株对 的影响也表明该属的丰度与肠道中 TMA 浓度之间存在关系。因此，这些变化的机制可能是肠道微生物群的调节。这些 菌株可能具有缓解与 TMAO 相关疾病的治疗潜力。
