Sulaimani Medicolegal Institute, Qanat Street, Kurdistan Regional Government, Sulaimani, Sulaymaniyah 46001, Iraq.
School of Chemistry, Joseph Banks Laboratories, University of Lincoln, Lincoln LN6 7DL, UK.
Molecules. 2022 Mar 11;27(6):1826. doi: 10.3390/molecules27061826.
Strychnine (STN) and its major metabolite Strychnine N-Oxide (SNO) were examined electrochemically. Both parent compounds and its major metabolite showed electroactivity on glassy carbon electrodes using CV and DPV techniques. One oxidation peak at 1008 mV was observed for STN with the optimum peak intensity at pH 7. SNO produced two oxidation peaks, at 617 mV and 797 mV, at pH 5. The peaks demonstrated irreversible behaviour and the irreversibility of the system was confirmed at different scan rates. A calibration curve was produced for both CV and DPV measurements and the sensitivity of the proposed EC method was good compared with previous electrochemical and non-electrochemical methods. The precision of oxidation peak of STN using the STN-MIP method produced a maximum value of 11.5% and 2.32% for inter-day and intraday %RSD, respectively. The average% recovery was around 92%. The electrochemical method has been successfully applied to the determination of STN in spiked plasma and urine samples. For SNO, both anodic peaks of SNO demonstrated irreversible behaviour. A different sweep rate was used for calculating the number of ‘transfer electrons’ in the system; based on this, the mechanism of oxidation reaction was proposed. Calibration curves for both oxidative peaks were produced using DPV measurements. The second anodic peak demonstrated high linearity and precision with %RSD < 1.96%.
番木鳖碱（STN）及其主要代谢产物番木鳖碱 N-氧化物（SNO）进行了电化学研究。使用 CV 和 DPV 技术，在玻碳电极上，母体化合物及其主要代谢产物均显示出电化学活性。STN 在 pH 7 时观察到 1008 mV 的一个氧化峰，最佳峰强度。SNO 在 pH 5 时产生两个氧化峰，分别为 617 mV 和 797 mV。这些峰表现出不可逆行为，在不同的扫描速率下证实了体系的不可逆性。为 CV 和 DPV 测量制作了校准曲线，与以前的电化学和非电化学方法相比，所提出的 EC 方法的灵敏度较好。使用 STN-MIP 方法，STN 氧化峰的精密度产生了 11.5%和 2.32%的最大值，分别为日内和日间%RSD。平均%回收率约为 92%。该电化学方法已成功应用于加标血浆和尿液样品中 STN 的测定。对于 SNO，SNO 的两个阳极峰均表现出不可逆行为。使用不同的扫速计算体系中的“转移电子”数；基于此，提出了氧化反应的机理。使用 DPV 测量制作了两个氧化峰的校准曲线。第二个阳极峰表现出高线性和精密度，%RSD<1.96%。
