Department of Chemistry, Faculty of Science, Shahid Beheshti University, Evin, Tehran, Iran.
Bioelectrochemistry. 2012 Dec;88:164-70. doi: 10.1016/j.bioelechem.2012.03.007. Epub 2012 Apr 13.
The electrochemical behavior of raloxifene (RLX) on the surface of a glassy carbon electrode (GCE) has been studied by cyclic voltammetry (CV). The CV studies were performed in various supporting electrolytes, wide range of potential scan rates, and pHs. The results showed an adsorption-controlled and quasi-reversible process for the electrochemical reaction of RLX, and a probable redox mechanism was suggested. Under the optimum conditions, differential pulse voltammetry (DPV) was applied for quantitative determination of the RLX in pharmaceutical formulations. The DPV measurements showed that the anodic peak current of the RLX was linear to its concentration in the range of 0.2-50.0μM with a detection limit of 0.0750μM, relative standard deviation (RSD %) below 3.0%, and a good sensitivity. The proposed method was successfully applied for determination of the RLX in pharmaceutical and human plasma samples with a good selectivity and suitable recovery.
采用循环伏安法（CV）研究了雷洛昔芬（RLX）在玻碳电极（GCE）表面的电化学行为。CV 研究在各种支持电解质、宽电位扫描速率和 pH 值范围内进行。结果表明，RLX 的电化学反应是受吸附控制的准可逆过程，并提出了可能的氧化还原机制。在最佳条件下，采用差分脉冲伏安法（DPV）定量测定药物制剂中的 RLX。DPV 测量表明，RLX 的阳极峰电流与其在 0.2-50.0μM 范围内的浓度呈线性关系，检测限为 0.0750μM，相对标准偏差（RSD%）低于 3.0%，灵敏度良好。该方法成功应用于药物和人血浆样品中 RLX 的测定，具有良好的选择性和合适的回收率。
