特定丝氨酸蛋白酶快速降解 SARS-CoV-2 刺突 S 蛋白。
Rapid Degradation of SARS-CoV-2 Spike S Protein by A Specific Serine Protease.
机构信息
Biochemistry Department, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
Laboratory of Medical Biology Center, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
出版信息
Molecules. 2022 Mar 14;27(6):1882. doi: 10.3390/molecules27061882.
The S protein of SARS-CoV-2 is a crucial structural and functional component for virus entry. Due to the constant mutation of the virus, there are very limited ways to prevent and control COVID-19. This experiment used a macroscopic SDS-PAGE method and proved that the S protein of wild-type SARS-CoV-2 virus, especially the S1 subunit, is very sensitive to alkaline serine protease with acidic pI (ASPNJ), NJ represents (Izuka) from which ASP is purified). ASPNJ cleaves proteins when the carbonyl group of the peptide bond is contributed by arginine or lysine. ASPNJ can degrade the S protein very quickly and effectively in vitro with relative selectivity. It can be inferred that the S, S1 and RBD of SARS-CoV-2 variants can also be easily degraded by ASPNJ. This rapid and strong degradation of the S protein by ASPNJ may become a potential new treatment strategy.
SARS-CoV-2 的 S 蛋白是病毒进入的关键结构和功能组成部分。由于病毒的不断突变，预防和控制 COVID-19 的方法非常有限。本实验采用宏观 SDS-PAGE 方法，证明野生型 SARS-CoV-2 病毒的 S 蛋白，特别是 S1 亚基，对具有酸性 pI（ASPNJ）的碱性丝氨酸蛋白酶非常敏感，NJ 代表（Izuka），其中 ASP 被纯化）。ASPNJ 可在体外以相对选择性快速有效地切割由精氨酸或赖氨酸提供肽键羰基的蛋白质。可以推断，SARS-CoV-2 变体的 S、S1 和 RBD 也可以被 ASPNJ 轻易降解。ASPNJ 对 S 蛋白的快速和强烈降解可能成为一种潜在的新治疗策略。
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