Laure Nana Nguefang, Ahn Juhee
Department of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea.
Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 24341, Korea.
Pathogens. 2022 Mar 13;11(3):349. doi: 10.3390/pathogens11030349.
This study was designed to assess the effect of β-lactam/β-lactamase inhibitor combinations on the inhibition of biofilm formation of Typhimurium. The anti-planktonic and anti-biofilm activities of ampicillin (AMP), ceftriaxone (CEF), and combination treatments of antibiotics and sulbactam (AMP + SUL and CEF + SUL) were evaluated against antibiotic-sensitive . Typhimurium ATCC 19585 (ST) and clinically isolated multidrug-resistant (MDR) . Typhimurium CCARM 8009 (ST). Compared to the control, the minimum inhibitory concentrations (MICs) of AMP against ST and CEF against ST were decreased from 32 to 16 μg/mL and 0.25 to 0.125 μg/mL, respectively, in the presence of SUL. The numbers of ST treated with AMP + SUL and CEF + SUL were effectively reduced by more than 2 logs after 4 h of incubation at 37 °C. The β-lactamase activities of ST and ST treated with AMP and CEF were reduced from 3.3 to 2.6 μmol/min/mL and from 8.3 to 3.4 μmol/min/mL, respectively, in the presence of SUL. The biofilm cell numbers of ST and ST were reduced at all treatments after 24 h of incubation at 37 °C. The biofilm cell numbers of ST and ST were reduced by more than 2 logs in the presence of SUL compared to the AMP and CEF alone. The lowest relative fitness level was 0.6 in ST treated with AMP + SUL, while no significant differences in the relative fitness were observed in ST. This study suggests that β-lactamase inhibitors (BLIs) could be used for controlling biofilm formation of β-lactamase-producing multidrug-resistant . Typhimurium.
本研究旨在评估β-内酰胺/β-内酰胺酶抑制剂组合对鼠伤寒沙门氏菌生物膜形成的抑制作用。针对抗生素敏感的鼠伤寒沙门氏菌ATCC 19585（ST）和临床分离的多重耐药（MDR）鼠伤寒沙门氏菌CCARM 8009（ST），评估了氨苄西林（AMP）、头孢曲松（CEF）以及抗生素与舒巴坦联合治疗（AMP + SUL和CEF + SUL）的抗浮游菌和抗生物膜活性。与对照组相比，在舒巴坦存在的情况下，AMP对ST的最低抑菌浓度（MIC）从32 μg/mL降至16 μg/mL，CEF对ST的MIC从0.25 μg/mL降至0.125 μg/mL。在37℃孵育4小时后，用AMP + SUL和CEF + SUL处理的ST菌数有效减少了2个对数以上。在舒巴坦存在的情况下，ST以及用AMP和CEF处理的ST的β-内酰胺酶活性分别从3.3 μmol/分钟/毫升降至2.6 μmol/分钟/毫升，从8.3 μmol/分钟/毫升降至3.4 μmol/分钟/毫升。在37℃孵育24小时后，所有处理组的ST生物膜细胞数均减少。与单独使用AMP和CEF相比，在舒巴坦存在的情况下，ST的生物膜细胞数减少了2个对数以上。用AMP + SUL处理的ST的最低相对适合度水平为0.6，而ST的相对适合度未观察到显著差异。本研究表明，β-内酰胺酶抑制剂（BLIs）可用于控制产β-内酰胺酶的多重耐药鼠伤寒沙门氏菌的生物膜形成。
