Department of Gastroenterology, Ruijin Hospital North, Shanghai Jiaotong University School of Medicine, Shanghai, 201801, P.R. China.
Department of Microbiology, Bengbu Medical College, Bengbu, Anhui, 233030, P.R. China.
Sci Rep. 2020 Feb 20;10(1):3042. doi: 10.1038/s41598-020-59456-8.
Salmonella typhimurium is a pathogenic gram-negative bacterium, which is found primarily in the intestinal lumen. It often causes diarrhea in infants and young children and leads to food poisoning. Drug resistance of Salmonella typhimurium presented serious complications in clinical patients. In this study, we investigated the antibiotic susceptibility of Salmonella typhimurium standard strain L forms to the third and forth generation cephalosporins, in order to control and eliminate Salmonella typhimurium L forms in infection treatment. Salmonella typhimurium L forms were induced by β-lactam antibiotic cefazolin in the culture medium of bacterial L forms. The antibiotic susceptibility of Salmonella typhimurium L forms was analyzed by K-B drug susceptibility testing. The change trend of drug susceptibility and resistance of Salmonella typhimurium L forms was obtained in accordance with USA clinical and laboratory standards institute (CLSI) evaluation data and statistical analysis. Drug resistance of Salmonella typhimurium L forms showed little increasing trend compared with their parent bacteria. The L form inhibition zone was smaller than in the parent bacteria. However, the drug susceptibility of L forms of Salmonella typhimurium to the third and forth generation cephalosporins remained sensitive.The antibiotic susceptibility of Salmonella typhimurium L forms to the third and forth generation cephalosporins remains sensitive, and the combined use of multi-antibiotics is a convenient and effective method to reduce Salmonella typhimurium L forms occurrence.
鼠伤寒沙门氏菌是一种致病性革兰氏阴性菌，主要存在于肠道腔中。它常引起婴幼儿腹泻，并导致食物中毒。鼠伤寒沙门氏菌的耐药性在临床患者中引起了严重的并发症。在本研究中，我们调查了鼠伤寒沙门氏菌标准株 L 型对第三代和第四代头孢菌素的抗生素敏感性，以控制和消除感染治疗中的鼠伤寒沙门氏菌 L 型。在细菌 L 型培养基中，用β-内酰胺类抗生素头孢唑林诱导鼠伤寒沙门氏菌 L 型。采用 K-B 药敏试验分析鼠伤寒沙门氏菌 L 型的抗生素敏感性。根据美国临床和实验室标准协会（CLSI）评估数据和统计分析，得出鼠伤寒沙门氏菌 L 型的药敏性和耐药性变化趋势。与亲本细菌相比，鼠伤寒沙门氏菌 L 型的耐药性呈略有增加趋势。L 型的抑制带小于亲本细菌。然而，鼠伤寒沙门氏菌 L 型对第三代和第四代头孢菌素的药物敏感性仍然敏感。鼠伤寒沙门氏菌 L 型对第三代和第四代头孢菌素的抗生素敏感性仍然敏感，联合使用多种抗生素是减少鼠伤寒沙门氏菌 L 型发生的一种方便有效的方法。
