Demoncheaux Jean-Paul, Medkour Hacene, Louni Meriem, Laugier Laurie, Pasqualini Christelle, Fenollar Florence, Davoust Bernard, Mediannikov Oleg
Animal Epidemiology Expert Group of the Military Health Service, 37100 Tours, France.
Military Health Service, French Armed Forces in Senegal, Dakar 18524, Senegal.
Microorganisms. 2022 Feb 22;10(3):489. doi: 10.3390/microorganisms10030489.
Bartonellae are bacteria associated with mammals and their ectoparasites. Rodents often host different species of . The aim of this study was to investigate the presence of spp. in African giant pouched rats () and their ectoparasites in Dakar, Senegal. In 2012, 20 rats were caught, and their fleas were identified. DNA was extracted from 170 selected fleas and qPCR was carried out to detect spp. Subsequently, a culture was performed from the rat blood samples and the isolated strains (, , and ITS3) were genotyped. A total of 1117 fleas were collected from 19 rats and identified as , the tropical rat flea. DNA was detected in 148 of 170 selected fleas (87.1%). In addition, strains were isolated from the blood of 17 rats (85%). According to gene-sequence-based criteria for species definition, the isolated strains were identified as . (four strains) and two potential new species related to the zoonotic . . In this paper, these potentially new species are provisionally called Bartonella militaris (11 strains) and Bartonella affinis (two strains) until their description has been completed. and its fleas could constitute a public health risk in Dakar due to the high prevalence of infection reported.
巴尔通体是与哺乳动物及其体外寄生虫相关的细菌。啮齿动物常常携带不同种类的……本研究的目的是调查在塞内加尔达喀尔的非洲巨囊鼠及其体外寄生虫中巴尔通体属物种的存在情况。2012年，捕获了20只大鼠，并鉴定了它们身上的跳蚤。从170只选定的跳蚤中提取DNA，并进行定量聚合酶链反应以检测巴尔通体属物种。随后，对大鼠血液样本进行培养，并对分离出的菌株（、、和ITS3）进行基因分型。从19只大鼠身上共收集到1117只跳蚤，鉴定为印鼠客蚤，即热带鼠蚤。在170只选定的跳蚤中，有148只（87.1%）检测到巴尔通体DNA。此外，从17只大鼠（85%）的血液中分离出巴尔通体菌株。根据基于基因序列的物种定义标准，将分离出的菌株鉴定为……（4株）以及与动物源性巴尔通体相关的两个潜在新物种……在本文中，这些潜在的新物种在完成描述之前暂称为军事巴尔通体（11株）和亲近巴尔通体（2株）。由于所报告的巴尔通体感染的高流行率，非洲巨囊鼠及其跳蚤在达喀尔可能构成公共卫生风险。
