Thongwai Narumol, Futui Wirapong, Ladpala Nanthiwa, Sirichai Benjamat, Weechan Anuwat, Kanklai Jirapat, Rungsirivanich Patthanasak
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Research Center in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Microorganisms. 2022 Feb 28;10(3):528. doi: 10.3390/microorganisms10030528.
Bacterial cellulose (BC), a biopolymer, is synthesized by BC-producing bacteria. Almost all producing strains are classified in the family . In this study, bacterial strain P285 was isolated from contaminated honey wine in a honey factory in northern Thailand. Based on 16S rRNA gene sequence identification, the strain P285 revealed 99.8% identity with LMG 1529 . P285 produced the maximum BC production at 20-30 °C and an initial media pH of 9.0. The highest BC production in modified mineral salt medium (MSM) was exhibited when glucose (16%, /) and yeast extract (3.2%, /) were applied as carbon and nitrogen sources, respectively. When sugarcane (8-16%, /) or honey (ratio of honey to water = 1: 4) supplemented with yeast extract was used, the BC production was greater. The characterization of BC synthesized by P285 was undertaken using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectrometry. Meanwhile, X-ray diffraction results confirmed the presence of crystalline cellulose (2θ = 18.330, 21.390 and 22.640°). The maximum temperature of BC degradation was observed at 314 °C. Tensile properties analysis of hydrated and dried BC showed breaking strength of 1.49 and 0.66 MPa, respectively. These results demonstrated that P285 has a high potential for BC production especially when grown in high initial media pH. Therefore, the strain would be suitable as an agent to make BC, the value-added product in the related factories.
细菌纤维素（BC）是一种生物聚合物，由产BC的细菌合成。几乎所有的生产菌株都归类于 科。在本研究中，从泰国北部一家蜂蜜工厂受污染的蜂蜜酒中分离出细菌菌株P285。基于16S rRNA基因序列鉴定，菌株P285与LMG 1529的相似度为99.8%。P285在20-30°C和初始培养基pH值为9.0时产生的BC产量最高。当分别使用葡萄糖（16%，/）和酵母提取物（3.2%，/）作为碳源和氮源时，在改良矿物盐培养基（MSM）中BC产量最高。当使用添加酵母提取物的甘蔗（8-16%，/）或蜂蜜（蜂蜜与水的比例 = 1:4）时，BC产量更高。使用扫描电子显微镜（SEM）和傅里叶变换红外（FTIR）光谱对P285合成的BC进行了表征。同时，X射线衍射结果证实了结晶纤维素的存在（2θ = 18.330、21.390和22.640°）。观察到BC降解的最高温度为314°C。水合和干燥BC的拉伸性能分析表明，断裂强度分别为1.49和0.66 MPa。这些结果表明，P285在生产BC方面具有很高的潜力，尤其是在初始培养基pH值较高的情况下生长时。因此，该菌株适合作为相关工厂生产增值产品BC的原料。
