Cielecka Izabela, Ryngajłło Małgorzata, Maniukiewicz Waldemar, Bielecki Stanisław
Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, 90-573 Łódź, Poland.
Institute of General and Ecological Chemistry, Lodz University of Technology, 90-924 Łódź, Poland.
Polymers (Basel). 2021 Dec 19;13(24):4455. doi: 10.3390/polym13244455.
A new strain of bacteria producing cellulose was isolated from Kombucha and identified as , named SI1. In static conditions, the strain synthesises bacterial nanocellulose with an improved ability to stretch. In this study, utilisation of various carbon and nitrogen sources and the impact of initial pH was assessed in terms of bacterial nanocellulose yield and properties. SI1 produces cellulose efficiently in glycerol medium at pH 5.0-6.0 with a yield of 3.20-3.60 g/L. Glucose medium led to the synthesis of membrane characterised by a strain of 77%, which is a higher value than in the case of another species. Supplementation of medium with vitamin C results in an enhanced porosity and improves the ability of bacterial nanocellulose to stretch (up to 123%). The properties of modified membranes were studied by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and mechanical tests. The results show that bacterial nanocellulose produced in SH medium and vitamin C-supplemented medium has unique properties (porosity, tensile strength and strain) without changing the chemical composition of cellulose. The method of production BNC with altered properties was the issue of Polish patent application no. P.431265.
从康普茶中分离出一种新的产纤维素细菌菌株，鉴定为，命名为SI1。在静态条件下，该菌株合成的细菌纳米纤维素拉伸能力有所提高。在本研究中，从细菌纳米纤维素产量和性能方面评估了各种碳源和氮源的利用情况以及初始pH值的影响。SI1在pH值为5.0 - 6.0的甘油培养基中能高效产生纤维素，产量为3.20 - 3.60 g/L。葡萄糖培养基导致合成的膜的菌株比例为77%，这一数值高于另一种菌株的情况。在培养基中添加维生素C会增加孔隙率，并提高细菌纳米纤维素的拉伸能力（高达123%）。通过扫描电子显微镜、傅里叶变换红外光谱、X射线衍射和力学测试研究了改性膜的性能。结果表明，在SH培养基和添加维生素C的培养基中产生的细菌纳米纤维素具有独特的性能（孔隙率、拉伸强度和应变），而纤维素的化学成分不变。生产具有改变性能的细菌纳米纤维素的方法是波兰专利申请号为P.431265的专利主题。
