Atykyan Nelli, Revin Victor, Shutova Vitalina
National Research Mordovia State University, Saransk, Russia.
AMB Express. 2020 May 3;10(1):84. doi: 10.1186/s13568-020-01020-8.
Raman and Fourier Transform Infrared (FT-IR) spectroscopy was used for investigation of structural differences of bacterial celluloses (BC), obtained by cultivation native and immobilized cells of Gluconacetobacter sucrofermentans during static and dynamic regimes of cultivation on a molasses media. It was found that the Raman and FT-IR spectra could characterized the groups of the cellulose molecules. The culturing bacterial cellulose in the presence of results in an increase of crystalline and it increased during cultivated on a molasses media with the addition of 1.5% ethanol-75.62%. The degree of BC crystallinity increased during dynamic regime of cultivation is higher than under static regime one. The maximal BC content was observed when 0.5% ascorbic acid was added to the cultivation medium with molasses and native cells. It was found, the degree of BC crystallinity during static regime cultivation on a molasses medium with ethanol, increased significantly to 73.5%, and during dynamic regime-75.6%. So, in this study, the changes of the bacterial cellulose conformation of were revealed during bacterial cultivation in a medium containing molasses in various cultivation modes.
拉曼光谱和傅里叶变换红外（FT-IR）光谱用于研究细菌纤维素（BC）的结构差异，这些细菌纤维素是通过在糖蜜培养基上静态和动态培养条件下，培养天然和固定化的嗜糖醋酸杆菌细胞而获得的。研究发现，拉曼光谱和FT-IR光谱可以表征纤维素分子的基团。在存在[此处原文缺失相关内容]的情况下培养细菌纤维素会导致结晶度增加，并且在添加1.5%乙醇 - 75.62%的糖蜜培养基上培养时结晶度会进一步增加。在动态培养条件下BC的结晶度增加程度高于静态培养条件。当向含有糖蜜和天然细胞的培养基中添加0.5%抗坏血酸时，观察到BC的最大含量。研究发现，在含有乙醇的糖蜜培养基上静态培养期间，BC的结晶度显著增加至73.5%，而在动态培养条件下为75.6%。因此，在本研究中，揭示了在不同培养模式下，在含有糖蜜的培养基中细菌培养期间细菌纤维素构象的变化。
