Li Haozhou, Xia Yushan, Tian Zhenyang, Jin Yongxin, Bai Fang, Cheng Zhihui, Swietnicki Wieslaw, Wu Weihui, Pan Xiaolei
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China.
Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. R. Weigla 12, 53-114 Wroclaw, Poland.
Microorganisms. 2022 Mar 21;10(3):666. doi: 10.3390/microorganisms10030666.
Carbon metabolism plays an important role in bacterial physiology and pathogenesis. The type III secretion system (T3SS) of is a virulence factor that contributes to acute infections. It has been demonstrated that bacterial metabolism affects the T3SS. Meanwhile, expression of T3SS genes is negatively regulated by the small RNAs RsmY and RsmZ. In this study, we studied the relationship between the dihydrolipoamide acetyltransferase gene and the T3SS. Our results reveal an upregulation of RsmY and RsmZ in the mutant, which represses the expression of the T3SS genes. Meanwhile, the mutant is more tolerant to hydrogen peroxide. We demonstrate that the expression levels of the catalase KatB and the alkyl hydroperoxide reductase AhpB are increased in the mutant. The simultaneous deletion of and in the mutant restored the expression levels of and , as well as bacterial susceptibility to hydrogen peroxide. Thus, we identify a novel role of AceF in the virulence and oxidative response of .
碳代谢在细菌生理学和发病机制中起着重要作用。[细菌名称]的III型分泌系统（T3SS）是一种有助于急性感染的毒力因子。已经证明细菌代谢会影响T3SS。同时，T3SS基因的表达受到小RNA RsmY和RsmZ的负调控。在本研究中，我们研究了二氢硫辛酰胺乙酰转移酶基因[基因名称]与T3SS之间的关系。我们的结果显示，在[基因名称]突变体中RsmY和RsmZ上调，这抑制了T3SS基因的表达。同时，[基因名称]突变体对过氧化氢更具耐受性。我们证明在[基因名称]突变体中过氧化氢酶KatB和烷基过氧化氢还原酶AhpB的表达水平增加。在[基因名称]突变体中同时缺失[基因名称]和[基因名称]可恢复[基因名称]和[基因名称]的表达水平，以及细菌对过氧化氢的敏感性。因此，我们确定了AceF在[细菌名称]的毒力和氧化应激反应中的新作用。
