Selenium Nanoparticles Biosynthesized by and Their Effects on Cellular and Physiological Parameters in the Rainbow Trout .

The applications of nanoparticles (Nps) as food additives, health enhancers, and antimicrobials in animal production are increasing. The aim of this study was to evaluate the effect of selenium (Se) nanoparticles (SeNps) stabilized with L-cysteine (SeNps/L-Cys), as a nutritional supplement, on immunological, oxidative status, and productive parameters in TEM and SEM-EDS showed the accumulation of spherical SeNps entirely composed by elemental selenium (Se) as intracellular and extracellular deposits in UC-32 strain. The in vitro antioxidant capacity of SeNps/L-Cys was significant more efficient ROS scavengers than SeNps and NaSeO. We also evaluate the effect of SeNps/L-Cys on cell viability and oxidative stress in RTgill-W1, RTS-11, or T-PHKM cell lines. SeNps/L-Cys showed less toxic and high antioxidant activity than SeNps and NaSeO. Finally, the dietary SeNps/L-Cys had a significant better effect on both plasma lysozyme and respiratory burst activity (innate immune response), on tissular Gpx activity (oxidative status), and on well-being (productive parameter) of when it is compared to SeNps and NaSeO. SeNps/L-Cys is a promising alternative for nutritional supplement for with better performance than NaSeO and SeNps, ease to implementation, and reduced environmental impact.