Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, 1 Alzeraa Street, 44511, Zagazig City, Sharkia Province, Egypt.
Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, 1 Alzeraa Street, 44511, Zagazig City, Sharkia Province, Egypt.
Fish Shellfish Immunol. 2019 Nov;94:280-287. doi: 10.1016/j.fsi.2019.09.019. Epub 2019 Sep 6.
As recently applicable, there are few studies on the impact of using nano-selenium (nano-Se) on varied fish species. Where nothing reachable focused on its impact on tilapias so, the present analysis evaluated the efficacy of using nano-Se in tilapias on immune response, antioxidant defense compared by conventional Se form. 480 O. niloticus fingerlings were haphazardly grouped firstly into three groups with four replicates of each. The control one (CT) was fed on a basal diet. The second and third one supplemented with 0.7 mg/kg Se and nano-Se respectively for ten weeks. At the start day of the ninth week, two replicates from each group were injected by Streptococcus iniae where, the remaining replicates stand without challenge. Enhancement of growth performance measurements were noted in nano-Se compared to Se or CT groups. Existed anemia in S. iniae tilapias became alleviated by using nano-Se that also, improves the alteration of leucogram induced by challenge. Elevation of aminotransferases, alkaline phosphatase, lactate dehydrogenase (ALT, AST, ALP and LDH) and creatinine in Se and CT challenged replicates that seemed nearly normal by using nano-Se. Usage of nano-Se showed more powerful antioxidant activities than Se. There were an expansion of immunoglobulin M, lysozymes, glutathione peroxidase, nitric oxide, superoxide dismutase and catalase (IgM, LYZ, GPx, NO, SOD, CAT) and their related gene expression in nano-Se with contrast in Se or CT challenged groups. Nile tilapias challenged by S. iniae disclosed substantial expansion in the percentage of mortality in CT challenged fish (93.33%), followed by the group supplemented with Se (73.33%), whereas the lowermost one at fish supplemented by nano-Se (26.66%). The mortalities have been stopped from the 5, 12 and 14 days in, nano-Se, Se and CT respectively. It can be concluded that using of Se 0.7 mg/kginduce immunosuppressive, antioxidant, liver and kidneys negative impact on tilapias where the same dose from nano-Se was more potent immunomodulating and antioxidant. Also it is attend in counteracting the serious impact induced by S. iniae challenge.
最近可应用的，对使用纳米硒（nano-Se）对不同鱼类物种的影响的研究较少。没有任何研究关注其对罗非鱼的影响，因此，本分析评估了在罗非鱼中使用纳米硒代替传统硒形式对免疫反应和抗氧化防御的效果。480 尾奥尼罗非鱼鱼苗首先随机分为三组，每组四个重复。对照组（CT）喂食基础饲料。第二组和第三组分别补充 0.7mg/kg 的硒和纳米硒，为期十周。在第九周的第一天，每组的两个重复注射鳗弧菌，其余的重复没有受到挑战。与硒或 CT 组相比，纳米硒组的生长性能测量得到了提高。鳗弧菌罗非鱼的贫血得到了缓解，纳米硒还改善了挑战引起的白细胞计数的变化。硒和 CT 组受挑战的重复样本中转氨酶、碱性磷酸酶、乳酸脱氢酶（ALT、AST、ALP 和 LDH）和肌酐升高，而使用纳米硒后似乎接近正常。纳米硒表现出比硒更强的抗氧化活性。免疫球蛋白 M、溶菌酶、谷胱甘肽过氧化物酶、一氧化氮、超氧化物歧化酶和过氧化氢酶（IgM、LYZ、GPx、NO、SOD、CAT）及其相关基因表达在纳米硒组中得到了扩展，而在硒或 CT 组中则有所扩展。鳗弧菌挑战的尼罗罗非鱼在 CT 组受挑战的鱼中死亡率显著增加（93.33%），其次是补充硒的组（73.33%），而补充纳米硒的组死亡率最低（26.66%）。纳米硒、硒和 CT 组分别在第 5、12 和 14 天停止死亡。可以得出结论，使用 0.7mg/kg 的硒会对罗非鱼产生免疫抑制、抗氧化、肝脏和肾脏的负面影响，而相同剂量的纳米硒则具有更强的免疫调节和抗氧化作用。此外，它还能抵抗鳗弧菌挑战引起的严重影响。
