2017 年意大利拉齐奥地区基孔肯雅热疫情期间献血者中无基孔肯雅病毒感染证据。
Lack of Evidence of Chikungunya Virus Infection among Blood Donors during the Chikungunya Outbreak in Lazio Region, Italy, 2017.
机构信息
Department of Infectious Diseases, Italian National Institute of Health, 00161 Rome, Italy.
National Blood Centre, Italian National Institute of Health, 00161 Rome, Italy.
出版信息
Viruses. 2022 Mar 16;14(3):619. doi: 10.3390/v14030619.
BACKGROUND
The latest European Chikungunya virus (CHIKV) outbreak occurred in Italy in 2017, in the municipalities of Anzio and Rome (Lazio Region), with a secondary outbreak in the Calabrian Region. Most CHIKV infections are symptomatic but about 15% of people who acquire the infection may be asymptomatic. A retrospective study was conducted with the aim of assessing the prevalence of recent/ongoing CHIKV infections on the blood donor population in the Lazio Region, during the 2017 outbreak (including in the period before it was detected).
METHODS
The study was conducted on 4595 plasma samples from donors who donated in 14 different Blood Establishments in the Lazio Region, in the period June-November 2017. A total of 389 of these samples were collected in provinces not affected by the outbreak and were used as negative controls. All samples were tested for IgM detection by the use of an ELISA test, and positive samples were tested for confirmation through the use of a PRNT. Molecular tests were performed on sera that were found to be IgM-positive or borderline.
RESULTS
A total of 41 (0.89%) blood donors tested positive for IgM. None of these positive IgM ELISA results was confirmed either by PRNT or by molecular tests.
CONCLUSIONS
Our study has shown no evidence of recent/ongoing CHIKV infection in blood donors of the affected area.
背景
最近一次欧洲基孔肯雅热病毒（CHIKV）疫情于 2017 年在意大利的安齐奥和罗马（拉齐奥地区）的市镇爆发，卡拉布里亚地区也爆发了二次疫情。大多数 CHIKV 感染是有症状的，但约 15%的感染者可能无症状。本回顾性研究旨在评估 2017 年疫情期间（包括疫情检测前）拉齐奥地区献血人群中近期/正在发生的 CHIKV 感染的流行率。
方法
在 2017 年 6 月至 11 月期间，对来自拉齐奥地区 14 个不同血库的 4595 份血浆样本进行了研究。其中 389 份样本来自未受疫情影响的省份，用作阴性对照。所有样本均采用 ELISA 试验检测 IgM ，阳性样本通过 PRNT 进行确认检测。对 IgM 阳性或边界值的血清进行分子检测。
结果
共有 41 名（0.89%）献血者 IgM 检测呈阳性。这些阳性的 IgM ELISA 结果均未通过 PRNT 或分子检测得到确认。
结论
本研究未发现受影响地区献血者存在近期/正在发生的 CHIKV 感染。
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