Ahmad Nisar, Muhammad Jan, Khan Khalil, Ali Wajid, Fazal Hina, Ali Mohammad, Rahman Latif-Ur, Khan Hayat, Uddin Muhammad Nazir, Abbasi Bilal Haider, Hano Christophe
Centre for Biotechnology and Microbiology, University of Swat, Swat, 19200, Pakistan.
Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar, 25120, Pakistan.
BMC Chem. 2022 Mar 25;16(1):20. doi: 10.1186/s13065-022-00816-y.
Prunella vulgaris is medicinally important plant containing high-valued chemical metabolites like Prunellin which belong to family Lamiaceae and it is also known as self-heal. In this research, calli culture were exposed to differential ratios of gold (Au) and silver (Ag) nanoparticles (1:1, 1:2, 1:3, 2:1 and 3:1) along with naphthalene acetic acid (2.0 mg NAA) to investigate its antimicrobial potential. A well diffusion method was used for antimicrobial properties.
Here, two concentrations (1 and 2 mg/6 µl) of all treated calli cultures and wild plants were used against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Bacillus atrophaeus, Bacillus subtilis, Agrobacterium tumefaciens, Erwinia caratovora and Candida albicans. Dimethyl sulfoxide (DMSO) and antibiotics were used as negative and positive controls. Here, the calli exposed to gold (Au) nanoparticles (NPs) and 2.0 mg naphthalene acetic acid (NAA) displayed the highest activity (25.7 mm) against Salmonella typhi than other extracts, which was considered the most susceptible species, while Agrobacterium tumefaciens and Candida albicans was the most resistance species. A possible mechanism of calli induced nanoparticles was also investigated for cytoplasmic leakage.
From the above data it is concluded that Prunella vulgaris is medicinally important plant for the development of anti-microbial drugs using nanotechnology and applicable in various pharmaceutical research.
夏枯草是一种具有重要药用价值的植物，含有如夏枯草素等高价值化学代谢产物，属于唇形科，也被称为自愈草。在本研究中，愈伤组织培养物暴露于不同比例的金（Au）和银（Ag）纳米颗粒（1:1、1:2、1:3、2:1和3:1）以及萘乙酸（2.0毫克NAA）中，以研究其抗菌潜力。采用琼脂扩散法测定抗菌性能。
在此，所有处理过的愈伤组织培养物和野生植物的两种浓度（1和2毫克/6微升）被用于对抗大肠杆菌、铜绿假单胞菌、伤寒沙门氏菌、萎缩芽孢杆菌、枯草芽孢杆菌、根癌土壤杆菌、胡萝卜软腐欧文氏菌和白色念珠菌。二甲基亚砜（DMSO）和抗生素用作阴性和阳性对照。在此，暴露于金（Au）纳米颗粒（NPs）和2.0毫克萘乙酸（NAA）的愈伤组织对伤寒沙门氏菌表现出最高活性（25.7毫米），高于其他提取物，伤寒沙门氏菌被认为是最敏感的物种，而根癌土壤杆菌和白色念珠菌是最具抗性的物种。还研究了愈伤组织诱导纳米颗粒导致细胞质泄漏的可能机制。
从上述数据可以得出结论，夏枯草是利用纳米技术开发抗菌药物的重要药用植物，适用于各种药物研究。
