State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; School of Chemical and Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; Nanjing IPE Institute of Green Manufacturing Industry, Nanjing 211100, China.
Carbohydr Polym. 2022 Jun 15;286:119281. doi: 10.1016/j.carbpol.2022.119281. Epub 2022 Feb 25.
Extraction of chitin from crustacean waste with acidic natural deep eutectic solvents (NADESs) is usually accompanied by degradation of chitin, which lowers the yield and molecular weight of product. Herein, this study proposed a eco-friendly and feasible route for effectively improving the yield and molecular weight of chitin by introducing N-acetyl-D-glucosamine into ternary NADESs. A high molecular weight chitin with molecular weight of 3.92 × 10 Da, purity of 90.2% and yield of 85.6% was obtained from crab shell. Compared with conventional acid/alkali and binary NADESs method, the maximum yield of chitin extracted by ChCl-G-FA2 was increased by 1.57 times and 1.39 times respectively. Molecular weight of chitin was 3.16 times that of acid/alkali method. Recycling performance evaluation revealed that the purity of chitin could still reach 80.4% after five cycles of NADESs. This study provided a eco-friendly utilization strategy for crustacean waste based on multifunctional NADESs.
从甲壳类废物中用酸性天然深共熔溶剂（NADESs）提取甲壳素通常伴随着甲壳素的降解，这会降低产物的产率和分子量。在此，本研究通过向三元 NADESs 中引入 N-乙酰-D-葡萄糖胺，提出了一种有效提高甲壳素产率和分子量的环保且可行的方法。从蟹壳中得到了一种分子量为 3.92×10 Da、纯度为 90.2%、产率为 85.6%的高分子量甲壳素。与传统的酸/碱和二元 NADESs 方法相比，ChCl-G-FA2 提取的甲壳素的最大产率分别提高了 1.57 倍和 1.39 倍。甲壳素的分子量是酸/碱法的 3.16 倍。回收性能评估表明，NADESs 重复使用 5 次后，甲壳素的纯度仍可达到 80.4%。本研究为基于多功能 NADESs 的甲壳类废物的环保利用提供了一种策略。
