Zhejiang Provincial Key Laboratory of Anesthesiology, Department of Anesthesiology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China.
Department of Gynecology and Obstetrics, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China.
Sci Data. 2022 Mar 25;9(1):106. doi: 10.1038/s41597-022-01225-5.
Spermatogenesis is an efficient, complex, and highly organized proliferation and differentiation process that relies on multiple factors including testosterone produced by the Leydig cells. Although the critical role played by testosterone in spermatogenesis is well recognized, the mechanism by which it works is still not completely understood, partially due to the inability to specifically and precisely monitor testosterone-dependent changes within developing germ cells. Here we present single-cell RNA sequencing data from10,983 adult rat testicular cells after the rats were treated with ethanedimethanesulfonate, which temporarily eliminates Leydig cells. The elimination and recovery of Leydig cells represented a complete testosterone depletion and restoration cycle. The dataset, which includes all developing germ cells from spermatogonia to spermatozoa, should prove useful for characterizing developing germ cells, their regulatory networks, and novel cell-specific markers. The dataset should be particularly useful for exploring the effects of the androgen environment on the regulation of spermatogenesis. As this is the first single-cell RNA-Seq dataset for rat testes, it can also serve as a reference for future studies.
精子发生是一个高效、复杂且高度组织化的增殖和分化过程，依赖于多种因素，包括睾丸间质细胞产生的睾酮。尽管睾酮在精子发生中的关键作用已得到广泛认可，但它的作用机制仍不完全清楚，部分原因是无法特异性和精确地监测发育中的精原细胞中依赖于睾酮的变化。在这里，我们展示了大鼠用乙二磺酸盐处理后 10983 个成年大鼠睾丸细胞的单细胞 RNA 测序数据，乙二磺酸盐可暂时消除睾丸间质细胞。睾丸间质细胞的消除和恢复代表了一个完整的睾酮耗竭和恢复周期。该数据集包括从精原细胞到精子的所有发育中的精原细胞，应该有助于描述发育中的精原细胞、它们的调控网络和新的细胞特异性标记物。该数据集对于探索雄激素环境对精子发生调控的影响特别有用。由于这是大鼠睾丸的第一个单细胞 RNA-Seq 数据集，它也可以作为未来研究的参考。
