NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China.
Clinical Research Center for Reproduction and Genetics in Hunan Province, Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, China.
Mol Cell Biochem. 2024 Aug;479(8):2017-2033. doi: 10.1007/s11010-023-04840-x. Epub 2023 Sep 2.
Spermatogenesis, a key part of the spermiation process, is regulated by a combination of key cells, such as primordial germ cells, spermatogonial stem cells, and somatic cells, such as Sertoli cells. Abnormal spermatogenesis can lead to azoospermia, testicular tumors, and other diseases related to male infertility. The application of single-cell RNA sequencing (scRNA-seq) technology in male reproduction is gradually increasing with its unique insight into deep mining and analysis. The data cover different periods of neonatal, prepubertal, pubertal, and adult stages. Different types of male infertility diseases including obstructive and non-obstructive azoospermia (NOA), Klinefelter Syndrome (KS), Sertoli Cell Only Syndrome (SCOS), and testicular tumors are also covered. We briefly review the principles and application of scRNA-seq and summarize the research results and application directions in spermatogenesis in different periods and pathological states. Moreover, we discuss the challenges of applying this technology in male reproduction and the prospects of combining it with other technologies.
精子发生是精子形成过程中的一个关键部分，它受到关键细胞（如原始生殖细胞、精原干细胞和体细胞，如支持细胞）的组合调控。异常的精子发生可能导致无精子症、睾丸肿瘤和其他与男性不育相关的疾病。单细胞 RNA 测序（scRNA-seq）技术在男性生殖中的应用随着其对深度挖掘和分析的独特见解而逐渐增加。这些数据涵盖了新生儿、青春期前、青春期和成年等不同阶段。研究涵盖了不同类型的男性不育疾病，包括梗阻性和非梗阻性无精子症（NOA）、克氏综合征（KS）、唯支持细胞综合征（SCOS）和睾丸肿瘤。我们简要回顾了 scRNA-seq 的原理和应用，并总结了不同时期和病理状态下精子发生的研究结果和应用方向。此外，我们还讨论了该技术在男性生殖中的应用挑战以及与其他技术相结合的前景。
