Yoo Young Jin, Ko Joo Hwan, Lee Gil Ju, Kang Jiwon, Kim Min Seok, Stanciu Stefan G, Jeong Hyeon-Ho, Kim Dae-Hyeong, Song Young Min
School of Electrical Engineering and Computer Science, Gwangju Institute of Science and Technology, Gwangju, 61005, Republic of Korea.
Department of Electronics Engineering, Pusan National University, Busan, 46241, Republic of Korea.
Adv Mater. 2022 May;34(21):e2110003. doi: 10.1002/adma.202110003. Epub 2022 Apr 12.
Bright-field imaging of nanoscale bioparticles is a challenging task for optical microscopy because the light-matter interactions of bioparticles are weak on conventional surfaces due to their low refractive index and small size. Alternatively, advanced imaging techniques, including near-field microscopy and phase microscopy, have enabled visualization and quantification of the bioparticles, but they require assistance of sophisticated/customized systems and post-processing with complex established algorithms. Here, a simple and fast immunoassay device, Gires-Tournois immunoassay platform (GTIP) is presented, which provides unique color dynamics in response to optical environment changes and thus enables the label-free bright-field imaging and facile quantification of bioparticles using conventional optical microscopy. Bioparticles on GTIP slow down the velocity of reflected light, leading to vivid color change according to the local particle density and maximizing chromatic contrast for high spatial distinguishability. The particle distribution and density on the surface of the resonator are readily analyzed through 2D raster-scanning-based chromaticity analysis. GTIP offers multiscale sensing capability for target analytes that possess different refractive indices and sizes.
对光学显微镜而言，纳米级生物粒子的明场成像颇具挑战性，因为生物粒子与光的相互作用在传统表面上较弱，这是由于其低折射率和小尺寸所致。另外，包括近场显微镜和相显微镜在内的先进成像技术，已能够实现生物粒子的可视化和定量分析，但它们需要复杂/定制系统的辅助以及使用复杂的既定算法进行后处理。在此，我们展示了一种简单快速的免疫分析装置——吉雷斯 - 图尔诺伊免疫分析平台（GTIP），它能根据光学环境变化提供独特的颜色动态变化，从而使用传统光学显微镜实现生物粒子的无标记明场成像和便捷定量分析。GTIP上的生物粒子会降低反射光的速度，根据局部粒子密度导致鲜明的颜色变化，并最大化色度对比度以实现高空间分辨能力。通过基于二维光栅扫描的色度分析，可轻松分析谐振器表面的粒子分布和密度。GTIP为具有不同折射率和尺寸的目标分析物提供多尺度传感能力。
