The UTokyo Center for Integrative Science of Human Behavior (CiSHuB), The University of Tokyo, 3-8-1, Komaba, Meguro-ku, Tokyo, Japan.
Laboratory for Brain Connectomics Imaging, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan.
Hum Brain Mapp. 2022 Jul;43(10):3184-3194. doi: 10.1002/hbm.25843. Epub 2022 Mar 26.
Resting-state functional connectivity (rs-FC) is widely used to examine the functional architecture of the brain, and the blood-oxygenation-level-dependent (BOLD) signal is often utilized for determining rs-FC. However, the BOLD signal is susceptible to various factors that have less influence on the cerebral blood flow (CBF). Therefore, CBF could comprise an alternative for determining rs-FC. Since acquisition duration is one of the essential parameters for obtaining reliable rs-FC, we investigated the effect of acquisition duration on CBF-based rs-FC to examine the reliability of CBF-based rs-FC. Nineteen participants underwent CBF scanning for a total duration of 50 min. Variance of CBF-based rs-FC within the whole brain and 13 large-scale brain networks at various acquisition durations was compared to that with a 50-min duration using the Levene's test. Variance of CBF-based rs-FC at any durations did not differ from that at a 50-min duration (p > .05). Regarding variance of rs-FC within each large-scale brain network, the acquisition duration required to obtain reliable estimates of CBF-based rs-FC was shorter than 10 min and varied across large-scale brain networks. Altogether, an acquisition duration of at least 10 min is required to obtain reliable CBF-based rs-FC. These results indicate that CBF-based resting-state functional magnetic resonance imaging (rs-fMRI) with more than 10 min of total acquisition duration could be an alternative method to BOLD-based rs-fMRI to obtain reliable rs-FC.
静息态功能连接（rs-FC）被广泛用于研究大脑的功能结构，而血氧水平依赖（BOLD）信号常被用于确定 rs-FC。然而，BOLD 信号易受多种因素的影响，这些因素对脑血流（CBF）的影响较小。因此，CBF 可以作为确定 rs-FC 的替代指标。由于采集时间是获得可靠 rs-FC 的重要参数之一，我们研究了采集时间对基于 CBF 的 rs-FC 的影响，以检验基于 CBF 的 rs-FC 的可靠性。19 名参与者进行了总共 50 分钟的 CBF 扫描。使用 Levene 检验比较了不同采集时间与 50 分钟采集时间的全脑和 13 个大规模脑网络的基于 CBF 的 rs-FC 的方差。任何时间的基于 CBF 的 rs-FC 的方差与 50 分钟采集时间的方差没有差异（p>.05）。关于每个大规模脑网络内 rs-FC 的方差，获得可靠的基于 CBF 的 rs-FC 估计所需的采集时间短于 10 分钟，并且因大规模脑网络而异。总的来说，至少需要 10 分钟的采集时间才能获得可靠的基于 CBF 的 rs-FC。这些结果表明，具有至少 10 分钟总采集时间的基于 CBF 的静息态功能磁共振成像（rs-fMRI）可能是基于 BOLD 的 rs-fMRI 的替代方法，可获得可靠的 rs-FC。
