Shao Junjun, Sun Shuai, Zhan Da, Pan Lipeng, Min Guangzong, Li Xiaobao, Huang Kailun, Chen Wei, Yang Likun, Liu Xiang-Yang, Lin Naibo
The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, 422 Siming Nan Road, Xiamen 361005, People's Republic of China.
The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, 422 Siming Nan Road, Xiamen 361005, People's Republic of China; State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 3888 Dong Nanhu Road, Changchun 130033, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jul 5;275:121177. doi: 10.1016/j.saa.2022.121177. Epub 2022 Mar 19.
As a kind of reactive oxygen species, peroxynitrite is related to various diseases closely such as cancer and neurodegenerative diseases. Constructing probes with highly specific ability and a wide linear detection range for peroxynitrite detection is crucial for understanding the pathogenesis of related diseases and optimizing treatments. In this work, we developed a novel luminescent ratiometric fluorescence nanoprobe (PC-CDs) based on carbon dots and phycocyanin. PC-CDs are constructed by amidation reaction between carbon dots and phycocyanin. The nanoprobe we obtained has a good ability of distinguishing peroxynitrite from other reactive oxygen species and interfering substances. Moreover, the linear range of the nanoprobe is 0.5-100 μM and the limit of detection is 0.5 μM when detecting peroxynitrite. In the spiked recovery experiments under phosphate buffered saline (PBS) environment, our nanoprobe has a good recovery performance and the recovery is 99% - 104%, which will be beneficial to the further development of peroxynitrite testing and the research progress of related diseases. Finally, we discuss the quenching mechanism of peroxynitrite for nanoprobe, and found that there is the combination of dynamic and static quenching in the quenching process.
作为一种活性氧，过氧亚硝酸盐与癌症和神经退行性疾病等多种疾病密切相关。构建对过氧亚硝酸盐具有高特异性和宽线性检测范围的探针对于理解相关疾病的发病机制和优化治疗至关重要。在这项工作中，我们基于碳点和藻蓝蛋白开发了一种新型的发光比率荧光纳米探针（PC-CDs）。PC-CDs是通过碳点与藻蓝蛋白之间的酰胺化反应构建而成。我们获得的纳米探针具有良好的区分过氧亚硝酸盐与其他活性氧和干扰物质的能力。此外，该纳米探针在检测过氧亚硝酸盐时的线性范围为0.5-100 μM，检测限为0.5 μM。在磷酸盐缓冲盐水（PBS）环境下的加标回收实验中，我们的纳米探针具有良好的回收性能，回收率为99% - 104%，这将有利于过氧亚硝酸盐检测的进一步发展以及相关疾病的研究进展。最后，我们探讨了过氧亚硝酸盐对纳米探针的猝灭机制，发现猝灭过程中存在动态猝灭和静态猝灭的结合。
