State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science, Guangxi Normal University, Guilin, 541004, China; Guilin Normal College, Guilin, 541199, China.
State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science, Guangxi Normal University, Guilin, 541004, China.
Talanta. 2021 Aug 15;231:122421. doi: 10.1016/j.talanta.2021.122421. Epub 2021 Apr 17.
Peroxynitrite (ONOO) is a series of basic biological oxidants involved in physiological and pathological processes. The detection of ONOO in biological systems has been challenging due to its extremely short half-life and low steady-state concentration. In this work, a ratiometric fluorescent nanoprobe for ONOO was constructed by coupling covalently of graphene quantum dots (GQDs) with cyanine 5.5 (Cy5.5). This nanoprobe (GQD-Cy5.5) could selectively accumulate in mitochondrial, appears two strong fluorescence emission peaks at 520 and 694 nm. In the presence of ONOO, the intensity of fluorescence emission peak at 520 nm increased and the intensity of fluorescence emission peak at 694 nm decreased. The ratio (F/F) of fluorescence intensity at two emission peaks had a good linear relationship with the concentration of ONOO in the range of 0-6.0 μM, and the detection limit was 0.03 μM. The excellent properties of the nanoprobe enable its applications in the ratiometric fluorescence imaging of endogenous ONOO in cell mitochondria.
过氧亚硝酸盐（ONOO）是参与生理和病理过程的一系列基本生物氧化剂。由于其半衰期极短且稳态浓度低，因此在生物系统中检测 ONOO 一直具有挑战性。在这项工作中，通过将石墨烯量子点（GQDs）与花青 5.5（Cy5.5）共价偶联，构建了用于 ONOO 的比率荧光纳米探针。该纳米探针（GQD-Cy5.5）可以选择性地在线粒体中积累，在 520 和 694nm 处显示出两个强荧光发射峰。在 ONOO 存在下，520nm 处荧光发射峰的强度增加，694nm 处荧光发射峰的强度降低。两个发射峰的荧光强度比（F/F）与 ONOO 的浓度在 0-6.0μM 范围内呈良好的线性关系，检测限为 0.03μM。该纳米探针具有优异的性能，可用于细胞线粒体中内源性 ONOO 的比率荧光成像。
