Department of Physiology, Faculty of Medicine, University of Granada, Granada, Andalucía,Spain.
PROmoting FITness and Health through Physical Activity Research Group (PROFITH), Sport and Health University Research Institute (iMUDS), Department of Physical Education and Sports, Faculty of Sport Sciences, University of Granada, Granada, Andalucía,Spain.
Int J Sport Nutr Exerc Metab. 2022 Mar 26;32(4):275-284. doi: 10.1123/ijsnem.2021-0332. Print 2022 Jul 1.
This study aimed to investigate the effects of different exercise training programs on fasting plasma levels of oxylipins, endocannabinoids (eCBs), and eCBs-like molecules in middle-aged sedentary adults. A 12-week randomized controlled trial was conducted using a parallel group design. Sixty-five middle-aged adults (40-65 years old) were randomly assigned to: (a) no exercise (control group), (b) concurrent training based on international physical activity recommendations (PAR group), (c) high-intensity interval training (HIIT group), and (d) HIIT together with whole-body electromyostimulation (HIIT + EMS group). Plasma levels of oxylipins, eCBs, and eCBs-like molecules were determined in plasma samples before and after the intervention using targeted lipidomics. Body composition was assessed through dual-energy X-ray absorptiometry, and dietary intake through a food frequency questionnaire and three nonconsecutive 24-hr recalls. The physical activity recommendations, HIIT, and HIIT-EMS groups showed decreased plasma levels of omega-6 and omega-3-derived oxylipins, and eCBs and eCBs-like molecules after 12 weeks (all Δ ≤ -0.12; all p < .05). Importantly, after Bonferroni post hoc corrections, the differences in plasma levels of omega-6 and omega-3 oxylipins were not statistically significant compared with the control group (all p > .05). However, after post hoc corrections, plasma levels of anandamide and oleoylethanolamide were increased in the physical activity recommendations group compared with the control group (anandamide: Δ = 0.05 vs. -0.09; oleoylethanolamide: Δ = -0.12 vs. 0.013, all p ≤ .049). In conclusion, this study reports that a 12-week exercise training intervention, independent of the modality applied, does not modify fasting plasma levels of omega-6 and omega-3 oxylipins, eCBs, and eCBs-like molecules in middle-aged sedentary adults.
本研究旨在探讨不同运动训练方案对中年久坐成年人空腹血浆中氧化脂类、内源性大麻素（eCBs）和 eCBs 类似物水平的影响。采用平行组设计进行了一项为期 12 周的随机对照试验。65 名中年成年人（40-65 岁）被随机分配到：（a）不运动（对照组）、（b）基于国际体力活动建议的同时训练（PAR 组）、（c）高强度间歇训练（HIIT 组）和（d）HIIT 与全身肌电刺激（HIIT+EMS 组）。干预前后使用靶向脂质组学测定血浆中氧化脂类、eCBs 和 eCBs 类似物的水平。通过双能 X 射线吸收法评估身体成分，通过食物频率问卷和三次非连续 24 小时回忆评估饮食摄入。体力活动建议、HIIT 和 HIIT+EMS 组在 12 周后显示出 ω-6 和 ω-3 衍生的氧化脂类和 eCBs 及 eCBs 类似物的血浆水平降低（所有 Δ ≤ -0.12；所有 p <.05）。重要的是，经过 Bonferroni 事后校正后，与对照组相比，ω-6 和 ω-3 氧化脂类的血浆水平差异无统计学意义（所有 p >.05）。然而，经过事后校正后，与对照组相比，体力活动建议组的花生四烯酸和油酰乙醇酰胺的血浆水平增加（花生四烯酸：Δ = 0.05 对 -0.09；油酰乙醇酰胺：Δ = -0.12 对 0.013，所有 p ≤.049）。综上所述，本研究报告称，为期 12 周的运动训练干预，无论应用何种方式，都不会改变中年久坐成年人空腹血浆中 ω-6 和 ω-3 氧化脂类、eCBs 和 eCBs 类似物的水平。
