Label-free in vivo assessment of brain mitochondrial redox states during the development of diabetic cognitive impairment using Raman spectroscopy.

Mitochondrial redox imbalance has been recognized as a unifying cause for diabetic cognitive impairment. Currently, a robust method for the in vivo assessment of brain mitochondrial redox imbalance is still lacking. Here, we conducted a spectral study to assess brain mitochondrial redox imbalance in the process of diabetic cognitive impairment by using label-free resonance Raman spectroscopy (RRS). Our findings showed that mitochondrial redox imbalance in cultured neurons and organotypic cortical slices exposed to high glucose were quantified by the reduction of Raman peak area at 750 cm and 1128 cm, which were also associated with synaptic injury and neuron apoptosis. Raman peak area at 750 cm and 1128 cm were also decreased in db/db mice at the age of 8, 16 and 24 weeks, and had a high correlation with the mitochondrial NAD/NADH redox couple. Of note, this mitochondrial redox imbalance occurred before measurable cognitive decline in 8-week-old diabetic mice, and might signal impending diabetic cognitive impairment. In summary, RRS-based mitochondrial redox states assay enabled the in vivo assessment of brain mitochondrial redox imbalance, and might provide an early indicator to enhance the prediction of diabetic cognitive impairment and inform on the response to therapies targeting mitochondrial redox imbalance.