Abramczyk Halina, Brozek-Pluska Beata, Kopec Monika, Surmacki Jakub, Błaszczyk Maciej, Radek Maciej
Laboratory of Laser Molecular Spectroscopy, Institute of Applied Radiation Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590 Lodz, Poland.
Department of Neurosurgery, Spine and Peripheral Nerve Surgery, University Hospital WAM-CSW, Medical University of Lodz, Zeromskiego 113, 91-647 Lodz, Poland.
Cancers (Basel). 2021 Feb 25;13(5):960. doi: 10.3390/cancers13050960.
To monitor redox state changes and biological mechanisms occurring in mitochondrial cytochromes in cancers improving methods are required. We used Raman spectroscopy and Raman imaging to monitor changes in the redox state of the mitochondrial cytochromes in ex vivo human brain and breast tissues at 532 nm, 633 nm, 785 nm. We identified the oncogenic processes that characterize human infiltrating ductal carcinoma (IDC) and human brain tumors: gliomas; astrocytoma and medulloblastoma based on the quantification of cytochrome redox status by exploiting the resonance-enhancement effect of Raman scattering. We visualized localization of cytochromes by Raman imaging in the breast and brain tissues and analyzed cytochrome vibrations at 750, 1126, 1337 and 1584 cm as a function of malignancy grade. We found that the concentration of reduced cytochrome becomes abnormally high in human brain tumors and breast cancers and correlates with the grade of cancer. We showed that Raman imaging provides additional insight into the biology of astrocytomas and breast ductal invasive cancer, which can be used for noninvasive grading, differential diagnosis.
为了监测癌症中线粒体细胞色素发生的氧化还原状态变化和生物学机制，需要改进方法。我们使用拉曼光谱和拉曼成像技术，在532纳米、633纳米、785纳米波长下监测离体人脑和乳腺组织中线粒体细胞色素氧化还原状态的变化。我们通过利用拉曼散射的共振增强效应，基于细胞色素氧化还原状态的量化，确定了表征人类浸润性导管癌（IDC）和人脑肿瘤（胶质瘤、星形细胞瘤和髓母细胞瘤）的致癌过程。我们通过拉曼成像观察了乳腺和脑组织中细胞色素的定位，并分析了750、1126、1337和1584厘米处细胞色素的振动随恶性程度分级的变化。我们发现，还原型细胞色素的浓度在人脑肿瘤和乳腺癌中异常升高，且与癌症分级相关。我们表明，拉曼成像为星形细胞瘤和乳腺导管浸润癌的生物学特性提供了额外的见解，可用于非侵入性分级和鉴别诊断。
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