Rahmani Parisa, Heidari Ghobad, Farahmand Fatemeh, Moradzadeh Alireza
Pediatric Gastroenterology and Hepatology Research Center, Children's Medical Center, Pediatric Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pediatric, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Ann Med Surg (Lond). 2022 Mar 15;76:103489. doi: 10.1016/j.amsu.2022.103489. eCollection 2022 Apr.
Non-invasive biomarkers, for the diagnosis of celiac disease, can reduce the need for biopsy, particularly in pediatric patients. The aim of this study was to investigate the levels tissue transglutaminase antibody (tTG) and plasma citrulline and its correlation with intestinal biopsy.
In this cross-sectional descriptive study, Pediatric patients with celiac disease referred to (XXX)were included. The patients underwent tTG antibody test along with plasma citrulline measurements using HPLC ((high performance liquid chromatography). Biopsy was performed in all the patients and clinical and demographic findings were recorded in a patient form. The data were statistically analyzed using SPSSv22.
Of 118 patients with celiac disease, the mean level of citrulline in patients was 17.48 ± 6.92 and the mean tTG titer was 183.17 ± 41.25. The two variables were inversely correlated with each other, p < 0.01. With an increase in Marsh levels, a significant reduction in citrulline levels and an increase in plasma tTG levels were seen, p < 0.01, respectively. The mean citrulline and tTG titer was not associated with gender and the age of the patients.
Our findings indicate that citrulline and tTG antibody titer are significant biomarkers for the diagnosis of celiac disease and the severity of intestinal atrophy among pediatric patients.
用于诊断乳糜泻的非侵入性生物标志物可减少活检需求，尤其是在儿科患者中。本研究的目的是调查组织转谷氨酰胺酶抗体（tTG）和血浆瓜氨酸水平及其与肠道活检的相关性。
在这项横断面描述性研究中，纳入了转诊至（XXX）的患有乳糜泻的儿科患者。患者接受了tTG抗体检测以及使用高效液相色谱法（HPLC）进行血浆瓜氨酸测量。对所有患者进行了活检，并以患者表格记录了临床和人口统计学结果。使用SPSSv22对数据进行统计学分析。
在118例乳糜泻患者中，患者的瓜氨酸平均水平为17.48±6.92，tTG平均滴度为183.17±41.25。这两个变量呈负相关，p<0.01。随着马什分级水平的增加，瓜氨酸水平显著降低，血浆tTG水平升高，p<0.01。瓜氨酸平均水平和tTG滴度与患者的性别和年龄无关。
我们的研究结果表明，瓜氨酸和tTG抗体滴度是诊断儿科患者乳糜泻和肠道萎缩严重程度的重要生物标志物。
