Stuhne-Sekalec L, Chudzik J, Stanacev N Z
J Biochem Biophys Methods. 1986 Aug;13(1):23-7. doi: 10.1016/0165-022x(86)90004-7.
Evidence for an association of both hydrophilic insulin and hydrophobic cyclosporin with liposomes prepared from egg yolk lecithin, cholesterol, and stearylamine (7:2:2.25 molar ratio) was obtained by Sepharose-4B gel filtration. The method used to prepare unilamellar liposomes containing 29.7 nmol cyclosporin and 2.3 nmol insulin per mu mol of liposomal lecithin is described.
通过琼脂糖-4B凝胶过滤获得了亲水性胰岛素和疏水性环孢素与由蛋黄卵磷脂、胆固醇和硬脂胺（摩尔比为7:2:2.25）制备的脂质体相关联的证据。描述了用于制备每微摩尔脂质体卵磷脂含有29.7纳摩尔环孢素和2.3纳摩尔胰岛素的单层脂质体的方法。
