The Single-Nucleotide Polymorphism of miR-27a and the Expression of miR-27a in -Related Diseases and the Correlation with the Traditional Chinese Medicine Syndrome.

AIMS

The study aims to explore the effects of the single-nucleotide polymorphism of miR-27a and its expression in -related diseases and the relationship between gastric pathology and traditional Chinese medicine (TCM).

METHODS

Subjects were classified into six histopathological groups and five TCM syndrome groups. All specimens underwent detection through rapid urease test and methylene blue staining. Histopathological characteristics were observed by hematoxylin-eosin. The expression of miR-27a and its genotype were, respectively, detected by Quantitative Real-Time PCR and direct sequencing.

RESULTS

promoted the malignant evolution of gastric mucosa and were involved in the formation of TCM syndrome. In -positive patients, the frequency of miR-27a CT genotype at the locus and its expression in the gastric cancer group were higher than those in other pathological groups. TCM syndrome had a close relationship with histopathological changes, and patients with spleen-qi deficiency syndrome had a higher risk of gastric cancer than other syndromes, regardless of infection.

CONCLUSION

The C allele at miR-27a locus may be an oncogene in gastric cancer. High levels of miR-27a could play an important role in gastric malignant evolution, especially cancerization. There is a certain connection between TCM syndrome and pathological changes of the gastric mucosa to some extent, where patients with SQD syndrome had a higher risk of GC.