Saito I, Moro I
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Sep;38(9):1547-52.
Colostrum contains a large number of cells and humoral defence factors that play an important role in the protection of neonates. While colostral cells have been characterized by immunohistochemical criteria, the function of these cells has been poorly understood. In this study, localization of immunoglobulins and other components in human colostral cells was examined by an immunofluorescence technique. The results obtained were as follows: Colostral cells reactive with polyvalent anti-human immunoglobulins were neutrophils and macrophages with a granular staining pattern. The predominant immunoglobulin in colostral cells was IgA, followed by IgM. A small amount of IgG was also found in these cells. The distribution of SC was similar to that of IgA and J-chain, suggesting the presence of secretory IgA in colostral cells. Coincidence of kappa chain and lambda chain in the same granules of neutrophils and macrophages suggests that these immunoglobulins were phagocytosed by neutrophils and macrophages. This conclusion was supported the results of the experiment which showed that incubation of peripheral blood neutrophils and monocytes with cell free colostrum resulted in the appearance of immunoglobulins in granules.
初乳含有大量细胞和体液防御因子，在保护新生儿方面发挥着重要作用。虽然初乳细胞已通过免疫组织化学标准进行了表征，但这些细胞的功能仍知之甚少。在本研究中，通过免疫荧光技术检测了人初乳细胞中免疫球蛋白和其他成分的定位。获得的结果如下：与多价抗人免疫球蛋白反应的初乳细胞是具有颗粒状染色模式的中性粒细胞和巨噬细胞。初乳细胞中主要的免疫球蛋白是IgA，其次是IgM。在这些细胞中也发现了少量的IgG。分泌片（SC）的分布与IgA和J链相似，表明初乳细胞中存在分泌型IgA。中性粒细胞和巨噬细胞相同颗粒中κ链和λ链的重合表明这些免疫球蛋白被中性粒细胞和巨噬细胞吞噬。这一结论得到了实验结果的支持，该实验表明，外周血中性粒细胞和单核细胞与无细胞初乳孵育后，颗粒中会出现免疫球蛋白。
