通过转移含有M6基因的质粒将M群A组链球菌转化为M+型。
Conversion of an M- group A streptococcus to M+ by transfer of a plasmid containing an M6 gene.
作者信息
Scott J R, Guenthner P C, Malone L M, Fischetti V A
出版信息
J Exp Med. 1986 Nov 1;164(5):1641-51. doi: 10.1084/jem.164.5.1641.
Abstract
An M28-derived group A streptococcal strain deleted for the gene encoding M protein was converted to M+ by introduction of a plasmid carrying emm6, the structural gene for type 6 M protein from strain D471. The reconstituted M+ strain, JRS2, resists phagocytosis in human blood and is opsonized by anti-M6 hyperimmune serum, but not by anti-M28 serum. Immunofluorescent microscopy and ELISA demonstrate the presence of M protein on its surface. In addition, JRS2 removes opsonic antibodies from hyperimmune rabbit sera generated by immunization with purified ColiM6 protein and with a synthetic amino-terminal peptide derived from M6. Immunization of rabbits with JRS2 generates opsonic anti-M6 antibodies. These results indicate that the cloned emm6 gene contains the information necessary to convert a phagocytosis-sensitive streptococcus to phagocytosis resistance. Furthermore, it also contains the determinants for M type specificity and those required to elicit opsonic antibodies. It thus appears to determine all the traits associated with M protein.
摘要
一株源自M28的A群链球菌缺失了编码M蛋白的基因，通过导入携带emm6的质粒而转变为M+型，emm6是D471菌株6型M蛋白的结构基因。重构的M+菌株JRS2在人血液中抵抗吞噬作用，并且被抗M6超免疫血清调理，但不被抗M28血清调理。免疫荧光显微镜检查和ELISA证明其表面存在M蛋白。此外，JRS2从用纯化的ColiM6蛋白和源自M6的合成氨基末端肽免疫产生的超免疫兔血清中去除调理抗体。用JRS2免疫兔子产生调理抗M6抗体。这些结果表明，克隆的emm6基因包含将对吞噬作用敏感的链球菌转变为抗吞噬作用所需的信息。此外，它还包含M型特异性的决定因素以及引发调理抗体所需的决定因素。因此，它似乎决定了与M蛋白相关的所有特征。
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