The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China; Shanghai Key Laboratory of Embryo Original Diseases Shanghai, China.
The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China; Shanghai Key Laboratory of Embryo Original Diseases Shanghai, China.
Reprod Biomed Online. 2022 May;44(5):923-933. doi: 10.1016/j.rbmo.2021.11.019. Epub 2021 Dec 1.
Could extracellular vesicle-derived long non-coding RNA (lncRNA) serve as promising circulating biomarkers for endometriosis?
To obtain novel diagnostic markers, 85 patients with endometriosis were enrolled as the endometriosis group and 86 unaffected participants as the control group. RNA sequencing was performed to identify extracellular vesicle-derived lncRNA that were differentially expressed between women with endometriosis (n = 5) and unaffected participants (n = 6). Messenger RNA and lncRNA sequences of the plasma extracellular vesicles were analysed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. lncRNA expression levels were further validated using quantitative reverse transcriptase polymerase chain reaction. Moreover, receiver operating characteristic curve analysis was performed to determine the diagnostic value of candidate lncRNA. Clinical features were correlated to the expression levels of candidate lncRNA.
It was found that 210 lncRNA were significantly dysregulated; among these, expression of LINC01569, RP3-399L15.2, FAM138B and CH507-513H4.6 was significantly decreased, whereas expression of RP11-326N17.2, KLHL7-AS1 and MIR548XHG was increased, in the plasma of patients with endometriosis. Combined expression level of RP3-399L15.2 and CH507-513H4.6 was used to distinguish patients with endometriosis from control participants; the results revealed a sensitivity of 80.00% and specificity of 85.45% at the cut-off point, and an area under the ROC curve of 0.9045. The findings demonstrated the potential of these two lncRNA as diagnostic biomarkers for endometriosis. Moreover, CH507-513H4.6 alone may be useful in detecting early-stage endometriosis lesions.
The combination of RP3-399L15.2 and CH507-513H4.6 may be a potential candidate for endometriosis biomarkers.
细胞外囊泡衍生的长非编码 RNA（lncRNA）能否作为子宫内膜异位症有前途的循环生物标志物？
为了获得新的诊断标志物，共纳入 85 例子宫内膜异位症患者作为子宫内膜异位症组，86 例无子宫内膜异位症患者作为对照组。对 5 例子宫内膜异位症患者和 6 例无子宫内膜异位症患者的血浆细胞外囊泡进行 RNA 测序，以鉴定差异表达的 lncRNA。使用基因本体论和京都基因与基因组百科全书分析对血浆细胞外囊泡的信使 RNA 和 lncRNA 序列进行分析。进一步使用定量逆转录聚合酶链反应验证 lncRNA 的表达水平。此外，还进行了受试者工作特征曲线分析以确定候选 lncRNA 的诊断价值。将临床特征与候选 lncRNA 的表达水平相关联。
发现 210 个 lncRNA 明显失调；其中，LINC01569、RP3-399L15.2、FAM138B 和 CH507-513H4.6 的表达明显降低，而 RP11-326N17.2、KLHL7-AS1 和 MIR548XHG 的表达则增加。在子宫内膜异位症患者的血浆中。RP3-399L15.2 和 CH507-513H4.6 的联合表达水平用于区分子宫内膜异位症患者和对照组患者；在截止点时，结果显示敏感性为 80.00%，特异性为 85.45%，ROC 曲线下面积为 0.9045。这些发现表明这两种 lncRNA 具有作为子宫内膜异位症诊断生物标志物的潜力。此外，CH507-513H4.6 单独检测可能有助于早期子宫内膜异位症病变的检测。
RP3-399L15.2 和 CH507-513H4.6 的组合可能是子宫内膜异位症生物标志物的潜在候选物。
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