Research Unit of Analysis and Process Applied to The Environment - APAE (UR17ES32) Higher Institute of Applied Sciences and Technology of Mahdia, University of Monastir, Monastir 5000, Tunisia; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina 98166, Italy.
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina 98166, Italy.
Environ Toxicol Pharmacol. 2022 May;92:103855. doi: 10.1016/j.etap.2022.103855. Epub 2022 Mar 25.
Despite the availability of analytic data, little is known about the toxicity of salicylic acid (SA) on aquatic non-target organisms. The present study aimed at evaluating the impact of SA through a short-term exposure of the Mediterranean mussel Mytilus galloprovincialis to five environmentally relevant concentrations of SA. A set of suitable biomarkers was applied at selected time-points on mussel digestive glands, including histological observations and expression of oxidative stress related genes. The obtained results showed a conspicuous hemocytic infiltration among mussel digestive tubules, as confirmed also by a flow cytometric approach that revealed an increase of halinocytes and granulocytes. Interestingly, a significant dose and time dependent decrease in the expression levels of oxidative stress related genes was found in mussels exposed to SA except for the glutathione S-transferase gene that was significantly up-regulated in a time-dependent manner confirming its important role against oxidant species and in the metabolism of pharmaceuticals.
尽管有分析数据，但对于水杨酸（SA）对水生非目标生物的毒性知之甚少。本研究旨在通过短时间暴露于 5 种环境相关浓度的 SA 来评估 SA 的影响。在贻贝消化腺中应用了一组合适的生物标志物，包括组织学观察和氧化应激相关基因的表达。获得的结果显示，贻贝消化管中的血细胞明显浸润，这也通过流式细胞术得到了证实，该方法显示了血淋巴细胞和粒细胞的增加。有趣的是，在暴露于 SA 的贻贝中，除了谷胱甘肽 S-转移酶基因外，与氧化应激相关的基因表达水平呈显著的剂量和时间依赖性下降，该基因呈时间依赖性显著上调，证实了其在对抗氧化剂和药物代谢中的重要作用。
