Wang Chao, Zhu Kexiao, Shi Pengfei, Ding Xilai, Zhang Shusheng
Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, People's Republic of China.
Chem Commun (Camb). 2022 Apr 12;58(30):4779-4782. doi: 10.1039/d2cc00078d.
We describe a simple aptamer assay for the rapid analysis of aflatoxin B1 (AFB1), a potently carcinogenic fungal metabolite. The original anti-AFB1 aptamer was rationally truncated, obtaining a short aptamer that displayed better performance. Different concentrations of AFB1 were detected by measuring the circular dichroism spectra of this short aptamer. The detection limit reached 0.6 nM, which was lower than that of the original aptamer (80 nM).
我们描述了一种用于快速分析黄曲霉毒素B1（AFB1，一种具有强烈致癌性的真菌代谢产物）的简单适配体检测方法。对原始的抗AFB1适配体进行合理截短，得到了性能更佳的短适配体。通过测量该短适配体的圆二色光谱来检测不同浓度的AFB1。检测限达到0.6 nM，低于原始适配体的检测限（80 nM）。
