Chen Su, Sun Bo, Shi Zhenying, Miao Xuexia, Li Haichao
Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Shanghai, China.
University of Chinese Academy of Sciences, Beijing, China.
Plant Cell Environ. 2022 Jun;45(6):1914-1929. doi: 10.1111/pce.14321. Epub 2022 Apr 11.
Brown planthopper (BPH) and blast disease jointly or individually cause big yield losses every year. To identify genes and metabolites with potential contributions to the dual resistance against both biotic-stress factors, we carried out a transcriptome and metabolome analysis for susceptible and resistant rice varieties after BPH and rice blast infestations. Coexpression network analysis identified a modular pattern that had the highest correlation coefficients (0.81) after the BPH and rice blast (-0.81) treatments. In total, 134 phenylpropanoid biosynthesis pathway-related genes were detected in this group. We found that the flavanone 3-hydroxylase gene (OsF3H) had opposite expression trends in response to BPH and rice blast infestations whereas the OsF3'H had similar expression patterns. Genetics analysis confirmed that the OsF3H gene knockdown lines demonstrated the opposite resistance phenotypes against BPH and rice blast, whereas the OsF3'H knockout lines enhanced rice resistance against both pests. Consistently, our metabolomics analysis identified the metabolite eriodictyol, one putative essential product of these two genes, that was more highly accumulated in the resistant rice variety of RHT than in the susceptible variety MDJ. This study highlights a useful strategy for identifying more genes and metabolites that have potential synergistic effects on rice against to multiple biotic stresses.
褐飞虱（BPH）和稻瘟病每年单独或共同造成巨大的产量损失。为了鉴定对这两种生物胁迫因子具有双重抗性潜在贡献的基因和代谢物，我们对感虫和抗病水稻品种在受到褐飞虱和稻瘟病侵染后进行了转录组和代谢组分析。共表达网络分析确定了一种模块模式，在褐飞虱和稻瘟病（-0.81）处理后具有最高的相关系数（0.81）。在该组中总共检测到134个与苯丙烷生物合成途径相关的基因。我们发现黄烷酮3-羟化酶基因（OsF3H）在应对褐飞虱和稻瘟病侵染时具有相反的表达趋势，而OsF3'H具有相似的表达模式。遗传学分析证实，OsF3H基因敲低系对褐飞虱和稻瘟病表现出相反的抗性表型，而OsF3'H基因敲除系增强了水稻对这两种害虫的抗性。一致地，我们的代谢组学分析鉴定出代谢物圣草酚，这是这两个基因的一种假定必需产物，在抗性水稻品种RHT中比在感病品种MDJ中积累得更多。本研究突出了一种有用的策略，用于鉴定更多对水稻抵御多种生物胁迫具有潜在协同效应的基因和代谢物。
