Institute of Biomedicine, Research Centre for Integrative Physiology and Pharmacology, University of Turkugrid.1374.1, Turku, Finland.
Clinical Microbiomics, Copenhagen, Denmark.
Microbiol Spectr. 2022 Apr 27;10(2):e0089321. doi: 10.1128/spectrum.00893-21. Epub 2022 Mar 28.
Diet and gut microbiota are known to modulate metabolic health. Our aim was to apply a metagenomics approach to investigate whether the diet-gut microbiota-metabolism and inflammation relationships differ in pregnant overweight and obese women. This cross-sectional study was conducted in overweight ( = 234) and obese ( = 152) women during early pregnancy. Dietary quality was measured by a validated index of diet quality (IDQ). Gut microbiota taxonomic composition and species diversity were assessed by metagenomic profiling (Illumina HiSeq platform). Markers for glucose metabolism (glucose, insulin) and low-grade inflammation (high sensitivity C-reactive protein [hsCRP], glycoprotein acetylation [GlycA]) were analyzed from blood samples. Higher IDQ scores were positively associated with a higher gut microbiota species diversity ( = 0.273, = 0.007) in obese women, but not in overweight women. Community composition (beta diversity) was associated with the GlycA level in the overweight women (= 0.04) but not in the obese. Further analysis at the species level revealed a positive association between the abundance of species Alistipes finegoldii and the GlycA level in overweight women (logfold change = 4.74, = 0.04). This study has been registered at ClinicalTrials.gov under registration no. NCT01922791 (https://clinicaltrials.gov/ct2/show/NCT01922791). We observed partially distinct diet-gut microbiota-metabolism and inflammation responses in overweight and obese pregnant women. In overweight women, gut microbiota community composition and the relative abundance of A. finegoldii were associated with an inflammatory status. In obese women, a higher dietary quality was related to a higher gut microbiota diversity and a healthy inflammatory status.
饮食和肠道微生物群被认为可以调节代谢健康。我们的目的是应用宏基因组学方法来研究超重和肥胖孕妇的饮食-肠道微生物群-代谢和炎症的关系是否不同。这项横断面研究在超重（n=234）和肥胖（n=152）孕妇的早孕期间进行。膳食质量通过验证的饮食质量指数（IDQ）进行测量。通过宏基因组谱（Illumina HiSeq 平台）评估肠道微生物群的分类组成和物种多样性。从血液样本中分析葡萄糖代谢（葡萄糖、胰岛素）和低度炎症（高敏 C 反应蛋白[hsCRP]、糖蛋白乙酰化[GlycA]）标志物。在肥胖女性中，较高的 IDQ 评分与较高的肠道微生物多样性呈正相关（r=0.273，P=0.007），但在超重女性中没有相关性。社区组成（β多样性）与超重女性的 GlycA 水平相关（r=0.04），但与肥胖女性无关。进一步在物种水平上的分析表明，在超重女性中，物种 Alistipes finegoldii 的丰度与 GlycA 水平呈正相关（logfold change=4.74，P=0.04）。本研究已在 ClinicalTrials.gov 注册，注册号为 NCT01922791（https://clinicaltrials.gov/ct2/show/NCT01922791）。我们观察到超重和肥胖孕妇的饮食-肠道微生物群-代谢和炎症反应部分不同。在超重女性中，肠道微生物群群落组成和 A. finegoldii 的相对丰度与炎症状态相关。在肥胖女性中，较高的膳食质量与较高的肠道微生物多样性和健康的炎症状态有关。
