Xu Zhiwei, Li Jiabin, Hong Lue, Zhang Yangli, Wang Chunyu, Yang Hailong, Zhao Lisha, Qiu Ping, Du Zhi, Wang Hui
Jinhua Academy, Zhejiang Chinese Medical University, Jinhua, China.
Second Clinical Medical School, Zhejiang Chinese Medical University, Hangzhou, China.
PLoS One. 2024 Dec 5;19(12):e0314335. doi: 10.1371/journal.pone.0314335. eCollection 2024.
Poor dietary habits have been associated with dysbiosis and microbial imbalance in pregnant women. Such imbalances can pose health risks during pregnancy. This study aimed to explore the impact of Polygonati Rhizoma on the gut microbiota of pregnant women through In vitro simulated fermentation. Interestingly, significant differences in microbial community richness and structure were found between the control and the treatment with Polygonati Rhizoma. Analysis of composition and variability indicated that the treatment with Polygonati Rhizoma group showed higher levels of Lactobacillus and Bifidobacterium, but lower levels of Parabacteroides and Lachnoclostridium. The study also investigated specific genera differences between groups using the co-occurrence network analysis and their correlations with microbial metabolites by the redundancy analysis (RDA), Mantel-test network heatmap, and heatmap highlighting the relationships among gut microbiota, short-chain fatty acids (SCFAs), and gases in the absence or presence of Polygonati Rhizoma supplementation. Functional predictions from BugBase phenotype prediction indicated changes in potentially pathogenic and aerobic bacteria in Polygonati Rhizoma supplementation. Overall, the findings provide valuable insights into the influence of Polygonati Rhizoma on the gut microbiota in pregnant women associated with poor dietary habits.
不良饮食习惯与孕妇肠道菌群失调和微生物失衡有关。这种失衡在孕期会带来健康风险。本研究旨在通过体外模拟发酵探索黄精对孕妇肠道微生物群的影响。有趣的是，在对照组和黄精处理组之间发现了微生物群落丰富度和结构的显著差异。成分和变异性分析表明，黄精处理组的乳酸杆菌和双歧杆菌水平较高，但副拟杆菌和瘤胃球菌属水平较低。该研究还通过共现网络分析研究了组间特定属的差异，并通过冗余分析（RDA）、Mantel检验网络热图以及突出有无黄精补充剂时肠道微生物群、短链脂肪酸（SCFAs）和气体之间关系的热图，研究了它们与微生物代谢产物的相关性。BugBase表型预测的功能预测表明，补充黄精后潜在致病菌和好氧菌发生了变化。总体而言，这些发现为黄精对饮食习惯不良的孕妇肠道微生物群的影响提供了有价值的见解。
