Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005302.
Two Gram-stain-negative, strictly aerobic bacteria, strains L1-7-SE and R6, isolated from marine red algae, were characterized. They shared 99.9 % 16S rRNA gene sequence similarity and a 100 % digital DNA-DNA hybridization (DDH) value, representing members of a single species. Cells of strains L1-7-SE and R6 were catalase- and oxidase-positive motile rods with a single polar flagellum. Strains L1-7-SE and R6 optimally grew at 30-35 °C, pH 7.0-8.0 and with 1.0-2.0 % (w/v) NaCl. Ubiquinone-10 was the sole isoprenoid quinone and C cyclo 8 and summed feature 8 (comprising C 7 and/or C 6) were detected as the major cellular fatty acids. The DNA G+C contents of strains L1-7-SE and R6 were both 61.62 mol%. The polar lipids of strain L1-7-SE consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and two unidentified polar lipids. Phylogenetic analyses based on 16S rRNA gene and 120 protein marker sequences revealed that strains L1-7-SE and R6 formed a phyletic lineage within the genus and they were most closely related to NL21 and KY 101 with both 98.8 % 16S rRNA gene sequence similarities. Digital DDH values between strain L1-7-SE and the type strains of and were 60.3 and 29.5 %, respectively. The phenotypic, chemotaxonomic and molecular features support that strains L1-7-SE and R6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is L1-7-SE (=KACC 19076=KCTC 92231=JCM 31802).
两株革兰氏阴性、严格需氧的细菌,L1-7-SE 和 R6 株,从海洋红藻中分离得到。它们的 16S rRNA 基因序列相似度为 99.9%,数字 DNA-DNA 杂交(DDH)值为 100%,代表单一物种的成员。L1-7-SE 和 R6 株的细胞为过氧化氢酶和氧化酶阳性的运动杆状菌,具有单一的极鞭毛。L1-7-SE 和 R6 株在 30-35°C、pH7.0-8.0 和 1.0-2.0%(w/v)NaCl 下最佳生长。泛醌-10 是唯一的异戊烯醌,检测到 C cyclo 8 和总和特征 8(包括 C 7 和/或 C 6)作为主要的细胞脂肪酸。L1-7-SE 和 R6 株的 DNA G+C 含量均为 61.62 mol%。L1-7-SE 株的极性脂由二磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、一种未鉴定的胺脂、一种未鉴定的磷脂和两种未鉴定的极性脂组成。基于 16S rRNA 基因和 120 种蛋白标记序列的系统发育分析表明,L1-7-SE 和 R6 株在 属内形成一个分支,与 和 NL21 和 KY 101 最为密切相关,它们的 16S rRNA 基因序列相似度均为 98.8%。L1-7-SE 株与 和 模式株之间的数字 DDH 值分别为 60.3%和 29.5%。表型、化学分类和分子特征支持 L1-7-SE 和 R6 株代表 属的一个新种,建议将其命名为 sp. nov。模式株为 L1-7-SE(=KACC 19076=KCTC 92231=JCM 31802)。