Feng Tingye, Kim Kyung Hyun, Jeong Sang Eun, Kim Wonyong, Jeon Che Ok
Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Department of Microbiology, Chung-Ang University, College of Medicine, Seoul 06974, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Jan;68(1):283-288. doi: 10.1099/ijsem.0.002498. Epub 2017 Nov 21.
A Gram-stain-negative, non-motile and aerobic bacterial strain, designated L1 8-17, was isolated from a marine alga, Porphyridium marinum, in South Korea. Cells of strain L1 8-17 were found to be oxidase- and catalase-positive cocci without flagella. Growth of strain L1 8-17 was observed at 20-40 °C (optimum, 37 °C), pH 6.0-10.0 (optimum, pH 7.0-8.0) and in the presence of 0-7 % (w/v) NaCl (optimum, 2-3 %). The isoprenoid quinone detected was only ubiquinone-10. Summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c) and C16 : 0 were detected as major cellular fatty acids. The major polar lipids of strain L1 8-17 consisted of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and an unidentified lipid. The G+C content of the genomic DNA was 59.3 mol%. Strain L1 8-17 was most closely related to Marimonas arenosa CAU 1311, Tropicibacter naphthalenivorans C02 and Donghicola eburneus SW-277 with 96.68, 96.68 and 96.60 % 16S rRNA gene sequence similarities, respectively, but the strain formed a phylogenetic lineage clearly distinct from them within the family Rhodobacteraceae. On the basis of phenotypic, chemotaxonomic and molecular properties, strain L1 8-17 represents a novel genus of the family Rhodobacteraceae, for which the name Aquicoccus porphyridii gen. nov., sp. nov. is proposed. The type strain of the type species is L1 8-17 (KACC 18806=JCM 31543).
从韩国的一种海洋藻类——海紫球藻（Porphyridium marinum）中分离出一株革兰氏阴性、无运动性的需氧细菌菌株，命名为L1 8-17。发现L1 8-17菌株的细胞为氧化酶和过氧化氢酶阳性的球菌，无鞭毛。在20-40°C（最适温度为37°C）、pH 6.0-10.0（最适pH为7.0-8.0）以及0-7%（w/v）NaCl（最适浓度为2-3%）的条件下观察到L1 8-17菌株的生长。检测到的类异戊二烯醌只有泛醌-10。总和特征8（由C18:1ω7c/C18:1ω6c组成）和C16:0被检测为主要的细胞脂肪酸。L1 8-17菌株的主要极性脂质由磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的氨基脂质、一种未鉴定的磷脂和一种未鉴定的脂质组成。基因组DNA的G+C含量为59.3 mol%。L1 8-17菌株与沙海单胞菌（Marimonas arenosa）CAU 1311、萘食嗜热杆菌（Tropicibacter naphthalenivorans）C02和埃氏东河菌（Donghicola eburneus）SW-277的16S rRNA基因序列相似性分别为96.68%、96.68%和96.60%，但该菌株在红杆菌科内形成了一个明显与它们不同的系统发育谱系。基于表型、化学分类和分子特性，L1 8-17菌株代表红杆菌科的一个新属，为此提出新属名为紫菜水球菌属（Aquicoccus porphyridii），新种名为紫菜水球菌（Aquicoccus porphyridii）。该模式种的模式菌株为L1 8-17（KACC 18806 = JCM 31543）。
