HIV-1 感染患者肺囊虫肺炎中的外周免疫表型和 T 细胞受体库。
Peripheral immune phenotypes and T cell receptor repertoire in pneumocystis pneumonia in HIV-1 infected patients.
机构信息
Beijing Institute of Hepatology, Beijing You An Hospital, Capital Medical University, Beijing 100069, China.
Department of Respiratory and Infectious Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China.
出版信息
Clin Immunol. 2022 Apr;237:108985. doi: 10.1016/j.clim.2022.108985. Epub 2022 Mar 25.
BACKGROUND
The host immune response to pneumocystis pneumonia (PCP) involves interactions among immune cell subsets and cytokines. However, the definite pathogenesis and immunological influences of P. jirovecii have not been fully elucidated.
METHODS
Mass cytometry and high-throughput sequencing of the T cell receptor (TCR) were used to profile the host immune response to human immunodeficiency virus-1 (HIV-1) and P. jirovecii infection.
RESULTS
Our findings demonstrated that patients with PCP showed different immune cell proportions when compared to healthy controls. Changes in cytokines were found after anti-PCP treatment, suggested that cytokines may play an important role in controlling the pathogen. Furthermore, PCP patients showed marked reduction of TCR repertoire diversity. The diversity of TCR repertoire was restored by the anti-PCP treatment.
CONCLUSION
In summary, we profiled the composition and characteristics of immune environment in response to HIV-1 and P. jirovecii infection, which may contribute to elucidating host immunity.
背景
宿主对肺孢子菌肺炎（PCP）的免疫反应涉及免疫细胞亚群和细胞因子之间的相互作用。然而，肺孢子菌的确切发病机制和免疫学影响尚未完全阐明。
方法
使用液质联用技术和 T 细胞受体（TCR）高通量测序来描绘人类免疫缺陷病毒-1（HIV-1）和肺孢子菌感染后宿主的免疫反应。
结果
我们的研究结果表明，与健康对照组相比，PCP 患者的免疫细胞比例不同。抗 PCP 治疗后发现细胞因子的变化，提示细胞因子可能在控制病原体方面发挥重要作用。此外，PCP 患者的 TCR 库多样性明显降低。抗 PCP 治疗可恢复 TCR 库的多样性。
结论
综上所述，我们对 HIV-1 和肺孢子菌感染的免疫环境组成和特征进行了描绘，这可能有助于阐明宿主免疫。
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