School of Applied Sciences, University of Campinas, Rua Pedro Zaccaria, 1300, Limeira, SP, 13484-350, Brazil.
School of Mechanical Engineering, University of Campinas, Rua Mendeley, 200, Campinas, SP, 13083-860, Brazil.
Sci Rep. 2022 Mar 28;12(1):5230. doi: 10.1038/s41598-022-09040-z.
Vascular graft surgeries are often conducted in trauma cases, which has increased the demand for scaffolds with good biocompatibility profiles. Biodegradable scaffolds resembling the extracellular matrix (ECM) of blood vessels are promising vascular graft materials. In the present study, polyurethane (PU) was blended with ECM proteins collagen and elastin (Col-El) and gelatin (Gel) to produce fibrous scaffolds by using the rotary jet spinning (RJS) technique, and their effects on in vitro properties were evaluated. Morphological and structural characterization of the scaffolds was performed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Micrometric fibers with nanometric rugosity were obtained. Col-El and Gel reduced the mechanical strength and increased the hydrophilicity and degradation rates of PU. No platelet adhesion or activation was observed. The addition of proteins to the PU blend increased the viability, adhesion, and proliferation of human umbilical vein endothelial cells (HUVECs). Therefore, PU-Col-El and PU-Gel scaffolds are promising biomaterials for vascular graft applications.
血管移植物手术常用于创伤情况，这增加了对具有良好生物相容性的支架的需求。类似于血管细胞外基质（ECM）的可生物降解支架是有前途的血管移植物材料。在本研究中，将聚氨酯（PU）与 ECM 蛋白胶原蛋白和弹性蛋白（Col-El）和明胶（Gel）混合，通过旋转射流纺丝（RJS）技术制备纤维支架，并评估其体外性能。使用扫描电子显微镜（SEM）和原子力显微镜（AFM）对支架进行形态和结构表征。得到了具有纳米粗糙度的微纤维。Col-El 和 Gel 降低了 PU 的机械强度，增加了其亲水性和降解速率。未观察到血小板黏附和激活。向 PU 共混物中添加蛋白质可提高人脐静脉内皮细胞（HUVEC）的活力、黏附和增殖。因此，PU-Col-El 和 PU-Gel 支架是有前途的血管移植物应用的生物材料。
