Webb Paige I, Hill Timothy J, Everett Nicholas A, Thornton Jade L, Cornish Jennifer L, Baracz Sarah J
Department of Psychology, C3A, Macquarie University, North Ryde, NSW, 2109, Australia.
Centre for Emotional Health, Macquarie University, North Ryde, NSW, 2109, Australia.
Psychopharmacology (Berl). 2022 Apr;239(4):1129-1141. doi: 10.1007/s00213-022-06103-x. Epub 2022 Mar 26.
Stress exposure during adolescence contributes to developing a methamphetamine (METH) use disorder. However, most of the studies investigating addiction-related behaviours include only male rodents, despite METH addiction rates being higher in females. Furthermore, animal studies investigating the effects of stress on methamphetamine addiction have used only basic self-administration models which may not be sensitive to the effects of stress.
This project explored whether adolescent isolation stress exposure increases the incidence of four key addiction-related behaviours in female rats.
Thirty-two female rat pups were caged in groups of four or individually during adolescence from postnatal (PND) day 22, with the latter being re-socialised in groups of four on PND 43. In adulthood, rats were tested for addiction-like behaviours in a METH self-administration paradigm modelling motivation to take METH, persistence in drug-seeking behaviour when METH was not available, resistance to extinction, and propensity to reinstate after a period of withdrawal.
Adolescent social isolation resulted in lower METH intake during acquisition; however, the paradigm modelling drug-seeking when the drug was unavailable engendered intermittent METH bingeing in all rats, abolishing the group differences in intake during this phase. Adolescent social isolation also accelerated extinction of non-reinforced lever pressing, and increased stress-primed reinstatement, compared to the group-housed rats.
Adolescent social isolation stress alters various methamphetamine addiction-like behaviours in female rats.
青春期遭受压力会促使甲基苯丙胺（冰毒）使用障碍的形成。然而，尽管女性的冰毒成瘾率更高，但大多数调查成瘾相关行为的研究仅纳入了雄性啮齿动物。此外，研究压力对甲基苯丙胺成瘾影响的动物研究仅使用了基本的自我给药模型，这些模型可能对压力的影响不敏感。
本项目探讨了青春期隔离应激暴露是否会增加雌性大鼠四种关键成瘾相关行为的发生率。
32只雌性幼鼠在出生后第22天（PND 22）的青春期被分成每组4只关在笼中或单独饲养，后者在PND 43时重新以每组4只进行群居饲养。成年后，在甲基苯丙胺自我给药范式中对大鼠进行成瘾样行为测试，该范式模拟了摄取甲基苯丙胺的动机、在无法获得甲基苯丙胺时持续的觅药行为
