Kavi Sidharthan V, Nagendran Krishnan, Baranwal V K
Division of Genetics and Tree Improvement, Institute of Forest Biodiversity (ICFRE), Hyderabad, India.
ICAR-Indian Institute of Vegetable Research, Varanasi, India.
Virus Genes. 2022 Jun;58(3):244-253. doi: 10.1007/s11262-022-01896-7. Epub 2022 Mar 26.
Transcriptome datasets available in public domain serve as valuable resource for identification and characterization of novel viral genomes. Poleroviruses are economically important plant-infecting RNA viruses belonging to the family Solemoviridae. In the present study, we explored the plant transcriptomes available in public domain and identified five putative novel poleroviruses tentatively named as Foeniculum vulgare polerovirus (FvPV), Kalanchoe marnieriana polerovirus (KmPV), Paspalum notatum polerovirus (PnPV), Piper methysticum polerovirus (PmPV), Trachyspermum ammi polerovirus (TaPV) and a novel enamovirus named as Celmisia lyallii enamovirus (ClEV) in Foeniculum vulgare, Kalanchoe marnieriana, Paspalum notatum, Piper methysticum, Trachyspermum ammi and Celmisia lyallii, respectively. Coding-complete genomes (5.56-5.74 kb) of CIEV, KmPV, PnPV, PmPV and TaPV were recovered while only the partial genome of FvPV could be recovered. The genome organization of identified viruses except ClEV is 5'-ORF0-ORF1-ORF2-ORF3a-ORF3-ORF4-ORF5-3' while that of ClEV is 5'-ORF0-ORF1-ORF2-ORF3-ORF5-3'. Phylogenetic analysis revealed that poleroviruses of apiaceous plants formed a monophyletic clade within the genus Polerovirus.
公共领域中可用的转录组数据集是鉴定和表征新型病毒基因组的宝贵资源。粉虱传双生病毒是属于独脚金病毒科的具有经济重要性的植物感染性RNA病毒。在本研究中，我们探索了公共领域中可用的植物转录组，并鉴定出五种推定的新型粉虱传双生病毒，暂命名为茴香粉虱传双生病毒（FvPV）、长寿花粉虱传双生病毒（KmPV）、雀稗粉虱传双生病毒（PnPV）、卡瓦胡椒粉虱传双生病毒（PmPV）、印度莳萝粉虱传双生病毒（TaPV），以及一种在茴香、长寿花、雀稗、卡瓦胡椒、印度莳萝和莱雅尔氏仙女木中分别鉴定出的新型蚕豆萎蔫病毒属病毒，命名为莱雅尔氏仙女木蚕豆萎蔫病毒（ClEV）。CIEV、KmPV、PnPV、PmPV和TaPV的编码完整基因组（5.56 - 5.74 kb）被成功获得，而仅获得了FvPV的部分基因组。除ClEV外，鉴定出的病毒的基因组结构为5'-ORF0-ORF1-ORF2-ORF3a-ORF3-ORF4-ORF5-3'，而ClEV的基因组结构为5'-ORF0-ORF1-ORF2-ORF3-ORF5-3'。系统发育分析表明，伞形科植物的粉虱传双生病毒在粉虱传双生病毒属内形成了一个单系分支。
