Palanga Essowè, Martin Darren P, Galzi Serge, Zabré Jean, Bouda Zakaria, Neya James Bouma, Sawadogo Mahamadou, Traore Oumar, Peterschmitt Michel, Roumagnac Philippe, Filloux Denis
Laboratoire Biosciences, Unité de Formation et de Recherche en Sciences de la Vie et de la Terre (UFR-SVT), Université Ouaga I Pr Joseph Ki-Zerbo, 03 BP 7021, Ouagadougou, Burkina Faso.
Laboratoire de Virologie et de Biotechnologies Végétales, INERA, 01 BP 476, Ouagadougou, Burkina Faso.
Arch Virol. 2017 Jul;162(7):2149-2152. doi: 10.1007/s00705-017-3327-z. Epub 2017 Mar 23.
The full-length genome sequences of two novel poleroviruses found infecting cowpea plants, cowpea polerovirus 1 (CPPV1) and cowpea polerovirus 2 (CPPV2), were determined using overlapping RT-PCR and RACE-PCR. Whereas the 5845-nt CPPV1 genome was most similar to chickpea chlorotic stunt virus (73% identity), the 5945-nt CPPV2 genome was most similar to phasey bean mild yellow virus (86% identity). The CPPV1 and CPPV2 genomes both have a typical polerovirus genome organization. Phylogenetic analysis of the inferred P1-P2 and P3 amino acid sequences confirmed that CPPV1 and CPPV2 are indeed poleroviruses. Four apparently unique recombination events were detected within a dataset of 12 full polerovirus genome sequences, including two events in the CPPV2 genome. Based on the current species demarcation criteria for the family Luteoviridae, we tentatively propose that CPPV1 and CPPV2 should be considered members of novel polerovirus species.
利用重叠RT-PCR和RACE-PCR技术，测定了两种新发现的感染豇豆植株的马铃薯卷叶病毒属病毒——豇豆马铃薯卷叶病毒1（CPPV1）和豇豆马铃薯卷叶病毒2（CPPV2）的全长基因组序列。5845个核苷酸的CPPV1基因组与鹰嘴豆褪绿矮缩病毒最为相似（同一性为73%），而5945个核苷酸的CPPV2基因组与菜豆温和黄化病毒最为相似（同一性为86%）。CPPV1和CPPV2基因组均具有典型的马铃薯卷叶病毒基因组结构。对推导的P1-P2和P3氨基酸序列进行的系统发育分析证实，CPPV1和CPPV2确实属于马铃薯卷叶病毒属病毒。在12个完整的马铃薯卷叶病毒属病毒基因组序列数据集中检测到4个明显独特的重组事件，其中包括在CPPV2基因组中的两个事件。根据黄症病毒科目前的种属划分标准，我们初步提议将CPPV1和CPPV2视为新的马铃薯卷叶病毒属病毒种的成员。
