Monteil H, Rasoamananjara D, Vetter M T
Pathol Biol (Paris). 1986 Jun;34(5 Pt 2):648-52.
The susceptibility to antibiotics of 100 Gram negative obligate anaerobes of the Bacteroides and Fusobacterium species was tested using the ATB ANA method. Results were compared to those obtained by addition of the tested antibiotics to Wilkins-Chalgren medium in the critical concentrations. Agreement between the two methods was satisfactory (80 to 100%) for the low cutoff concentrations, except for doxycycline. When the pathogens are divided into susceptible, intermediate and resistant strains, results are more difficult to interpret because of the existence of the intermediate category: however, major discrepancies (R/S) did not exceed 3.2% except for cefotaxime (15%). Our results show that use of ATB ANA microplates in an anaerobic enclosure is a valuable method in clinical practice.
采用ATB ANA方法检测了100株拟杆菌属和梭杆菌属革兰氏阴性专性厌氧菌对抗生素的敏感性。将结果与在临界浓度下将受试抗生素添加到威尔金斯-查尔格伦培养基中所获得的结果进行了比较。除强力霉素外,两种方法在低临界浓度下的一致性令人满意(80%至100%)。当将病原体分为敏感、中介和耐药菌株时,由于存在中介类别,结果更难解释:然而,除头孢噻肟(15%)外,主要差异(R/S)不超过3.2%。我们的结果表明,在厌氧环境中使用ATB ANA微孔板是临床实践中的一种有价值的方法。