von Meyenburg K, Hansen F G, Nielsin L D, Riise E
Mol Gen Genet. 1978 Apr 17;160(3):287-95. doi: 10.1007/BF00332972.
Specialized transducing phages lambda asn harboring chromosomal DNA and genetic markers on either side of the asn gene were isolated. Phages carrying chromosomal DNA counterclockwise of the asn gene can upon infection establish themselves as self-replicating plasmids in asn, recA hosts lysogenic for lambda. It is concluded that this bypassing of normal lambda immunity is due to the presence of the chromosomal replication origin, oriC, in this class of phages. Genetic analysis and the determination of restriction endonuclease cleavage patterns of the different lambda asn lead to the allocation of oriC within 1.5 megadaltons of the asn gene towards the uncA, uncB genes at 82 min on the genetic map of E. coli. The clockwise order of genes on the chromosomes is found to be: bglB, (pst, glmS), (uncA, uncB), oriC, asn, trkD, rbs, rrnC, ilv.
分离出了携带染色体DNA以及天冬酰胺酶基因（asn）两侧遗传标记的特异性转导噬菌体λasn。携带位于asn基因逆时针方向染色体DNA的噬菌体，在感染后可在对λ呈溶原状态的asn、recA宿主中建立起自我复制质粒。得出的结论是，这类噬菌体对正常λ免疫的绕过是由于存在染色体复制起点oriC。对不同的λasn进行遗传分析和限制性内切酶切割模式的测定，结果表明oriC在遗传图谱上位于大肠杆菌82分钟处，在asn基因朝向uncA、uncB基因的1.5兆道尔顿范围内。发现染色体上基因的顺时针顺序为：bglB、（pst、glmS）、（uncA、uncB）、oriC、asn、trkD、rbs、rrnC、ilv。
